Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (RNF144A Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNF144A antibody detected the RNF144A protein (arrow).)

Rabbit anti-Human RNF144A Polyclonal Antibody | anti-RNF144A antibody

RNF144A Antibody (Center)

Gene Names
RNF144A; RNF144; UBCE7IP4
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RNF144A; Polyclonal Antibody; RNF144A Antibody (Center); E3 ubiquitin-protein ligase RNF144A; 632-; RING finger protein 144A; UbcM4-interacting protein 4; Ubiquitin-conjugating enzyme 7-interacting protein 4; KIAA0161; RNF144; UBCE7IP4; anti-RNF144A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RNF144A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the Central region of human RNF144A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
156-184
Sequence Length
292
Applicable Applications for anti-RNF144A antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(RNF144A Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNF144A antibody detected the RNF144A protein (arrow).)

Western Blot (WB) (RNF144A Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNF144A antibody detected the RNF144A protein (arrow).)

Immunohistochemistry (IHC)

(RNF144A Antibody (Center) (MBS9212971)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF144A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (RNF144A Antibody (Center) (MBS9212971)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF144A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-RNF144A antibody
The protein encoded by this protein contains a RING
finger, a motif known to be involved in protein-DNA and
protein-protein interactions. The mouse counterpart of this protein
has been shown to interact with Ube2l3/UbcM4, which is an
ubiquitin-conjugating enzyme involved in embryonic development.
Product Categories/Family for anti-RNF144A antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Adkins, D.E., et al. Mol. Psychiatry (2010) In press :
Hoja, M.R., et al. Exp. Cell Res. 259(1):239-246(2000)
Martinez-Noel, G., et al. FEBS Lett. 454(3):257-261(1999)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32890
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF144A
NCBI Official Synonym Full Names
ring finger protein 144A
NCBI Official Symbol
RNF144A
NCBI Official Synonym Symbols
RNF144; UBCE7IP4
NCBI Protein Information
E3 ubiquitin-protein ligase RNF144A
UniProt Protein Name
E3 ubiquitin-protein ligase RNF144A
UniProt Gene Name
RNF144A
UniProt Synonym Gene Names
KIAA0161; RNF144; UBCE7IP4
UniProt Entry Name
R144A_HUMAN

NCBI Description

The protein encoded by this protein contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. The mouse counterpart of this protein has been shown to interact with Ube2l3/UbcM4, which is an ubiquitin-conjugating enzyme involved in embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF144A: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Belongs to the RBR family. RNF144 subfamily.

Protein type: Membrane protein, integral; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: Golgi apparatus; cytoplasmic vesicle membrane; integral to membrane; plasma membrane

Molecular Function: zinc ion binding; ligase activity

Biological Process: protein ubiquitination

Research Articles on RNF144A

Similar Products

Product Notes

The RNF144A rnf144a (Catalog #AAA9212971) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 156-184. The RNF144A Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF144A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the RNF144A rnf144a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF144A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.