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Western Blot (WB) (Western blot analysis of extracts from Jurkat cells using CCNB1IP1 antibody.)

Rabbit anti-Human CCNB1IP1 Polyclonal Antibody | anti-CCNB1IP1 antibody

CCNB1IP1 Antibody

Gene Names
CCNB1IP1; HEI10; C14orf18
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CCNB1IP1; Polyclonal Antibody; CCNB1IP1 Antibody; C14orf18; Chromosome 14 open reading frame 18; CIP1_HUMAN; Cyclin B1 interacting protein 1; cyclin B1 interacting protein 1; E3 ubiquitin protein ligase; Cyclin-B1-interacting protein 1; E3 ubiquitin-protein ligase CCNB1IP1; Enhancer of invasion 10; Gm288; HEI10; Human enhancer of invasion 10; mei4; anti-CCNB1IP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CCNB1IP1 antibody detects endogenous levels of total CCNB1IP1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
277
Applicable Applications for anti-CCNB1IP1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus
Tissue Specificity
Highly expressed in heart. Detected at intermediate levels in liver and kidney, and at low levels in placenta, brain and lung.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (82%), Bovine (91%), Horse (82%), Sheep (91%), Dog (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells using CCNB1IP1 antibody.)

Western Blot (WB) (Western blot analysis of extracts from Jurkat cells using CCNB1IP1 antibody.)

Immunofluorescene (IF)

(MBS9605472 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescene (IF) (MBS9605472 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-CCNB1IP1 antibody
Function: Ubiquitin E3 ligase that acts as a limiting factor for crossing-over during meiosis: required during zygonema to limit the colocalization of RNF212 with MutS-gamma-associated recombination sites and thereby establish early differentiation of crossover and non-crossover sites. Later, it is directed by MutL-gamma to stably accumulate at designated crossover sites. Probably promotes the dissociation of RNF212 and MutS-gamma to allow the progression of recombination and the implementation of the final steps of crossing over (By similarity). Modulates cyclin-B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis.
Subunit Structure: Interacts with CCNB1, UBE2L3 and NF2.
Post-translational Modifications: Ubiquitinated; autoubiquitinated. Phosphorylated by CDK1 on serine or threonine residues (in vitro).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 32 kD
Predicted: 32 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase CCNB1IP1
NCBI Official Synonym Full Names
cyclin B1 interacting protein 1
NCBI Official Symbol
CCNB1IP1
NCBI Official Synonym Symbols
HEI10; C14orf18
NCBI Protein Information
E3 ubiquitin-protein ligase CCNB1IP1
UniProt Protein Name
E3 ubiquitin-protein ligase CCNB1IP1
UniProt Gene Name
CCNB1IP1
UniProt Synonym Gene Names
C14orf18; HEI10

NCBI Description

HEI10 is a member of the E3 ubiquitin ligase family and functions in progression of the cell cycle through G(2)/M.[supplied by OMIM, Apr 2004]

Uniprot Description

Ubiquitin E3 ligase that acts as a limiting factor for crossing-over during meiosis: required during zygonema to limit the colocalization of RNF212 with MutS-gamma-associated recombination sites and thereby establish early differentiation of crossover and non-crossover sites. Later, it is directed by MutL-gamma to stably accumulate at designated crossover sites. Probably promotes the dissociation of RNF212 and MutS-gamma to allow the progression of recombination and the implementation of the final steps of crossing over (). Modulates cyclin-B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis.

Research Articles on CCNB1IP1

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Product Notes

The CCNB1IP1 ccnb1ip1 (Catalog #AAA9605472) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCNB1IP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCNB1IP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CCNB1IP1 ccnb1ip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCNB1IP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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