Western Blot (WB)
((0.1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CCL21 Polyclonal Antibody | anti-CCL21 antibody
Goat anti-CCL21 Antibody
Western Blot: Approx 16kDa band observed in Human Lymph Node lysates (calculated MW of 14.6kDa according to NP_002980.1). Recommended concentration: 0. 03-0.1ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19). [provided by RefSeq, Sep 2014]
Uniprot Description
CCL21: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9p13
Cellular Component: extracellular region; extracellular space; intracellular
Molecular Function: CCR chemokine receptor binding; CCR7 chemokine receptor binding; chemokine activity; chemokine receptor binding
Biological Process: cell maturation; cell-cell signaling; dendritic cell chemotaxis; establishment of T cell polarity; formation of immunological synapse; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; positive regulation of actin filament polymerization; positive regulation of cell adhesion mediated by integrin; positive regulation of cell-matrix adhesion; positive regulation of chemotaxis; positive regulation of dendritic cell antigen processing and presentation; positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of JNK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of pseudopodium formation; positive regulation of receptor-mediated endocytosis; release of sequestered calcium ion into cytosol; ruffle organization and biogenesis; T cell costimulation
Research Articles on CCL21
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Product Notes
The CCL21 ccl21 (Catalog #AAA423176) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CCL21 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCL21 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 16kDa band observed in Human Lymph Node lysates (calculated MW of 14.6kDa according to NP_002980.1). Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the CCL21 ccl21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QGCRKDRGAS KTG. It is sometimes possible for the material contained within the vial of "CCL21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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