Immunohistochemistry (IHC)
(Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)
Rabbit anti-Human CCL2 / MCP1 Polyclonal Antibody | anti-CCL2 antibody
Anti-CCL2 / MCP1 Antibody (aa62-89) IHC-plus
IF (1:10 - 1:50)
IHC-P (1:75)
WB (1:1000)
For long term storage store at -20 degree C.
Immunohistochemistry (IHC)
(Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)
Immunohistochemistry (IHC)
(Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)
Immunofluorescence (IF)
(Confocal immunofluorescence of CCL2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescence of CCL2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Immunofluorescence of CCL2 Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
Immunofluorescence (IF)
(Immunofluorescence of CCL2 Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
Western Blot (WB)
(Western blot of CCL2 Antibody in HeLa cell line lysates (35 ug/lane). CCL2 (arrow) was detected using the purified antibody.)
Western Blot (WB)
(Western blot of CCL2 Antibody in HeLa cell line lysates (35 ug/lane). CCL2 (arrow) was detected using the purified antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric of HeLa cells using CCL2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric of HeLa cells using CCL2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Uniprot Description
CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Chemokine
Chromosomal Location of Human Ortholog: 17q11.2-q12
Cellular Component: dendrite; endocytic vesicle; extracellular region; extracellular space; nerve terminal; perikaryon; perinuclear region of cytoplasm; rough endoplasmic reticulum; synapse
Molecular Function: CCR2 chemokine receptor binding; chemokine activity; heparin binding; protein kinase activity; receptor binding
Biological Process: aging; angiogenesis; astrocyte cell migration; cell adhesion; cell surface receptor linked signal transduction; cellular calcium ion homeostasis; cellular homeostasis; cellular protein metabolic process; cellular response to insulin stimulus; chemotaxis; cytokine and chemokine mediated signaling pathway; cytoskeleton organization and biogenesis; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; humoral immune response; inflammatory response; JAK-STAT cascade; leukocyte migration during inflammatory response; lipopolysaccharide-mediated signaling pathway; lymphocyte chemotaxis; macrophage chemotaxis; MAPKKK cascade; maternal process involved in parturition; maternal process involved in pregnancy; monocyte chemotaxis; negative regulation of angiogenesis; negative regulation of neuron apoptosis; neutrophil chemotaxis; organ morphogenesis; organ regeneration; positive regulation of cellular extravasation; positive regulation of collagen biosynthetic process; positive regulation of endothelial cell proliferation; positive regulation of GTPase activity; positive regulation of inflammatory response; positive regulation of leukocyte mediated cytotoxicity; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of synaptic transmission; positive regulation of T cell activation; positive regulation of tumor necrosis factor production; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of cell shape; response to activity; response to amino acid stimulus; response to antibiotic; response to bacterium; response to ethanol; response to gamma radiation; response to heat; response to hypoxia; response to mechanical stimulus; response to progesterone stimulus; response to vitamin B3; response to wounding; signal transduction; transforming growth factor beta receptor signaling pathway; unfolded protein response; vascular endothelial growth factor receptor signaling pathway; viral genome replication
Disease: Human Immunodeficiency Virus Type 1, Susceptibility To; Mycobacterium Tuberculosis, Susceptibility To; Neural Tube Defects
Research Articles on CCL2
Similar Products
Product Notes
The CCL2 ccl2 (Catalog #AAA2400253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCL2 / MCP1 Antibody (aa62-89) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCL2 / MCP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS). Flow Cytometry (1:10 - 1:50) IF (1:10 - 1:50) IHC-P (1:75) WB (1:1000). Researchers should empirically determine the suitability of the CCL2 ccl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL2 / MCP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
