Goat CCKBR Polyclonal Antibody | anti-CCKBR antibody

Goat anti-CCKBR Antibody

Cat. Num. AAA421039

• Gene Names: CCKBR; GASR; CCK-B; CCK2R
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CCKBR; Polyclonal Antibody; Goat anti-CCKBR Antibody; cholecystokinin B receptor; HGNC: 1571; CCK-B; GASR; CCK2 receptor; cholecystokinin-B receptor/gastrin receptor; gastrin receptor; gastrin\cholecystokinin brain receptor; CCK2R; CCKBR antibody; cholecystokinin B receptor antibody; HGNC: 1571 antibody; CCK-B antibody; GASR antibody; CCK2 receptor antibody; cholecystokinin-B receptor/gastrin receptor antibody; gastrin receptor antibody; gastrin\cholecystokinin brain receptor antibody; CCK2R antibody; anti-CCKBR antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: FDGDSDSDSQSRVRNQ
• Sequence Length: 447

• Applicable Applications for anti-CCKBR antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000.; ; Western blot: Approx 80kDa band observed in Mouse Pancreas and Rat Stomach lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1and 49.0kDa according to Rat NP_037297.1). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.; ; IHC: Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml. In paraffin embedded Human Pancreas expression of CCKBR is detected in islet cells, and not in surrounding acinar cells. Recommended concentration: 6ug/ml.
• Immunogen: Peptide with sequence C-FDGDSDSDSQSRVRNQ, from the internal region of the protein sequence according to NP_795344.1.
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Western Blot

(MBS421039 (1ug/ml) staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

Immunohistochemistry

(MBS421039 (5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Immunohistochemistry

(MBS421039 (6ug/ml) staining of paraffin embedded Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)

Immunohistochemistry

(MBS421039 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 28875799
• NCBI GeneID: 887
• NCBI Accession #: NP_795344.1
• NCBI GenBank Nucleotide #: NM_176875.3
• NCBI Official Full Name: gastrin/cholecystokinin type B receptor isoform 1
• NCBI Official Synonym Full Names: cholecystokinin B receptor
• NCBI Official Symbol: CCKBR
• NCBI Official Synonym Symbols: GASR; CCK-B; CCK2R
• NCBI Protein Information: gastrin/cholecystokinin type B receptor
• UniProt Protein Name: Gastrin/cholecystokinin type B receptor
• Protein Family: Gastrin/cholecystokinin type B receptor
• UniProt Gene Name: CCKBR
• UniProt Synonym Gene Names: CCKRB; CCK-B receptor; CCK-BR; CCK2-R
• UniProt Entry Name: GASR_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. Alternative splicing results in multiple transcript variants. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq, Dec 2015]

Uniprot Description

CCKBR: Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase regulator activity; cholecystokinin receptor activity; gastrin receptor activity; phosphoinositide phospholipase C activity; protein binding; type B gastrin/cholecystokinin receptor binding

Biological Process: cell proliferation; cell surface receptor linked signal transduction; digestion; elevation of cytosolic calcium ion concentration; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); gastric acid secretion; gland development; gut development; positive regulation of cell proliferation; regulation of phosphoinositide 3-kinase activity; sensory perception