Western Blot (WB)
((0.5ug/ml) staining of HEK293 (A) and MCF7 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CCAR2/DBC1 Polyclonal Antibody | anti-CCAR2 antibody
Goat anti-CCAR2/DBC1 (aa842-855) Antibody
Western Blot: Approx 140kDa band observed in nuclear lysates of cell lines HEK293 and MCF7 (calculated MW of 103kDa according to NP_066997.3). The observed molecular weight is explained by its acetylation (Zheng et al, Mol Cell Biol.2013 Dec;33(24): 4960-70. PMID: 24126058) Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of HEK293 (A) and MCF7 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of HEK293 (A) and MCF7 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of NIH3T3 nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of NIH3T3 nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
DBC-1: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Component of the DBIRD complex. Interacts with ZNF326/ZIRD; the interaction is direct. Interacts (via N-terminus) with SIRT1. Interacts (via N-terminus) with SUV39H1; this interaction abolishes the interaction with SIRT1. Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Apoptosis; Nuclear receptor co-regulator; Mitochondrial; Tumor suppressor
Chromosomal Location of Human Ortholog: 8p22
Cellular Component: cytoplasm; mitochondrial matrix; nuclear chromatin; nucleoplasm; nucleus; spliceosome
Molecular Function: enzyme binding; enzyme inhibitor activity; protein binding
Biological Process: cell cycle; mitochondrial fragmentation during apoptosis; mRNA processing; negative regulation of catalytic activity; negative regulation of cell growth; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of transcription, DNA-dependent; positive regulation of apoptosis; regulation of circadian rhythm; regulation of protein stability; regulation of RNA elongation; response to DNA damage stimulus; response to UV; rhythmic process; RNA splicing; transcription, DNA-dependent; Wnt receptor signaling pathway
Research Articles on CCAR2
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Product Notes
The CCAR2 ccar2 (Catalog #AAA423396) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CCAR2/DBC1 (aa842-855) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CCAR2/DBC1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 140kDa band observed in nuclear lysates of cell lines HEK293 and MCF7 (calculated MW of 103kDa according to NP_066997.3). The observed molecular weight is explained by its acetylation (Zheng et al, Mol Cell Biol.2013 Dec;33(24): 4960-70. PMID: 24126058) Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the CCAR2 ccar2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ESHNRFSATE VTNK. It is sometimes possible for the material contained within the vial of "CCAR2/DBC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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