Rabbit anti-Human CC50B Polyclonal Antibody | anti-CC50B antibody

CC50B, NT (TMEM30B, CDC50B, Cell cycle control protein 50B, Transmembrane protein 30B) (Azide free) (HRP)

Cat. Num. AAA6281314

• Gene Names: TMEM30B; CDC50B
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CC50B; Polyclonal Antibody; NT (TMEM30B; CDC50B; Cell cycle control protein 50B; Transmembrane protein 30B) (Azide free) (HRP); TMEM30B; Transmembrane protein 30B; anti-CC50B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CC50B antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CC50B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 50-80 from the N-terminal region of human CC50B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CC50B Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane). CC50B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CC50B Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CC50B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CC50B Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CC50B antibody

Antibodies; Transmembrane Proteins

References

Katoh, Y., et al. Oncol. Rep. 12(4):939-943(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 63003930
• NCBI GeneID: 161291
• NCBI Accession #: NP_001017970
• NCBI GenBank Nucleotide #: NM_001017970
• UniProt Accession #: Q3MIR4
• Molecular Weight: 39kDa
• NCBI Official Full Name: cell cycle control protein 50B
• NCBI Official Synonym Full Names: transmembrane protein 30B
• NCBI Official Symbol: TMEM30B
• NCBI Official Synonym Symbols: CDC50B
• NCBI Protein Information: cell cycle control protein 50B
• UniProt Protein Name: Cell cycle control protein 50B
• UniProt Gene Name: TMEM30B
• UniProt Synonym Gene Names: CDC50B
• UniProt Entry Name: CC50B_HUMAN

Uniprot Description

TMEM30B: Belongs to the CDC50/LEM3 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: lipid transport