Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat CBX5/HP1-Alpha Polyclonal Antibody | anti-CBX5 antibody
Goat anti-CBX5/HP1-Alpha, biotinylated Antibody
Western Blot: Approx 25kDa band observed in HeLa lysates (calculated MW of 22.2kDa according to NP_036249.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
HP1 alpha: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys- 9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: centric heterochromatin; chromocenter; histone deacetylase complex; histone methyltransferase complex; kinetochore; nuclear chromosome, telomeric region; nuclear envelope; nuclear heterochromatin; nucleolus; nucleoplasm; nucleus; PML body; transcriptional repressor complex
Molecular Function: chromatin binding; histone deacetylase binding; methylated histone residue binding; protein binding; protein binding, bridging; protein homodimerization activity
Biological Process: blood coagulation; negative regulation of transcription, DNA-dependent; viral reproduction
Research Articles on CBX5
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Product Notes
The CBX5 cbx5 (Catalog #AAA423571) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CBX5/HP1-Alpha, biotinylated Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CBX5/HP1-Alpha can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 25kDa band observed in HeLa lysates (calculated MW of 22.2kDa according to NP_036249.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the CBX5 cbx5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NKRKSNFSNS ADDIK. It is sometimes possible for the material contained within the vial of "CBX5/HP1-Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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