Rabbit CBLB Polyclonal Antibody | anti-CBLB antibody

CBLB Antibody

Cat. Num. AAA9404538

• Gene Names: CBLB; Cbl-b; RNF56; Nbla00127
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: CBLB; Polyclonal Antibody; CBLB Antibody; Cbl-b; RNF56; Nbla00127; anti-CBLB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total CBLB protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 982

• Applicable Applications for anti-CBLB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human CBLB.
• Target Name: CBLB
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CBLB antibody.)

Related Product Information for anti-CBLB antibody

The Casitas B lineage lymphoma (Cbl) proteins (in mammals these are c-Cbl, Cbl-b, and Cbl-c) are a family of single subunit RING finger protein-ubiquitin E3 ligases that contain multiple protein interaction motifs (1). All Cbl proteins have a highly conserved N-terminal tyrosine kinase-binding (TKB) domain that mediates interactions between Cbl proteins and phosphorylated tyrosine residues on Cbl substrates. C-terminal to the RING finger, Cbl proteins have proline-rich domains that mediate interactions with SH3 domain-containing proteins. Phosphorylated tyrosine residues mediate interactions with SH2 domain-containing proteins such as the p85 subunit of PI3K. These protein-protein interaction motifs allow Cbl family proteins to function as adaptor proteins (2). This adaptor function contributes to the E3-dependent activities of Cbl proteins by targeting specific substrates for ubiquitination and degradation. The adaptor function also contributes to non-E3-dependent activities, such as the recruitment of proteins involved in receptor tyrosine kinase internalization, localization of Cbl proteins to specific subcellular compartments, and activation of discrete signaling pathways (1).

Product Categories/Family for anti-CBLB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 54112420
• NCBI GeneID: 868
• NCBI Accession #: NP_733762.2
• NCBI GenBank Nucleotide #: NM_170662.3
• UniProt Accession #: Q13191; Q13192; Q13193; Q3LIC0; Q63Z43; Q8IVC5; A8K9S7; B7WNM4
• Molecular Weight: 86,582 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL-B
• NCBI Official Synonym Full Names: Cbl proto-oncogene B, E3 ubiquitin protein ligase
• NCBI Official Symbol: CBLB
• NCBI Official Synonym Symbols: Cbl-b; RNF56; Nbla00127
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL-B
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL-B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: CBLB
• UniProt Synonym Gene Names: RNF56
• UniProt Entry Name: CBLB_HUMAN

Uniprot Description

Cbl-b: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization. Interacts with SH3 domain-containing proteins LCK, CRK and SORBS1. Interacts with LCP2 and ZAP70. May interact with CBL. Interacts with SH3 domain-containing proteins VAV1, FYN, FGR, PLCG1, GRB2, CRKL, PIK3R1 and SH3KBP1/CIN85. Identified in heterotrimeric complexes with SH3KBP1/CIN85, CD2AP and ARHGEF7, where one CBLB peptide binds two copies of the other protein. Interacts with poly-ubiquitinated proteins. Dimerization is required for the binding of poly-ubiquitin, but not for the binding of mono-ubiquitin. Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Ubiquitin conjugating system; EC 6.3.2.-; Adaptor/scaffold; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 3q13.11

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; signal transducer activity; zinc ion binding; phosphotyrosine binding; ubiquitin-protein ligase activity; calcium ion binding; ligase activity

Biological Process: cell surface receptor linked signal transduction; T cell activation; positive regulation of protein catabolic process; negative regulation of T cell receptor signaling pathway; protein ubiquitination; negative regulation of alpha-beta T cell proliferation; immune response; NLS-bearing substrate import into nucleus; signal transduction; positive regulation of T cell anergy