Rabbit anti-Human CBL Polyclonal Antibody | anti-CBL antibody

CBL, CT (RING finger protein 55, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, Signal transduction protein CBL, E3 ubiquitin-protein ligase CBL,RNF55, CBL2, CBL) (APC)

Cat. Num. AAA6281113

• Gene Names: CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CBL; Polyclonal Antibody; CT (RING finger protein 55; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; Signal transduction protein CBL; E3 ubiquitin-protein ligase CBL; RNF55; CBL2; CBL) (APC); RING finger protein 55; anti-CBL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CBL antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CBL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CBL.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-CBL antibody

The CBL oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.

Product Categories/Family for anti-CBL antibody

Antibodies; Ubiquitin Ligases

References

Chandra, V., et al. J. Virol. 84(8):3857-3867(2010). Song, J.J., et al. Cell. Signal. 22(3):553-563(2010). Kim, H.S., et al. Immunity 32(2):175-186(2010). Truitt, L., et al. Cancer Res. 70(3):1141-1153(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 52426745
• NCBI GeneID: 867
• NCBI Accession #: NP_005179.2
• NCBI GenBank Nucleotide #: NM_005188.3
• UniProt Accession #: P22681; A3KMP8
• Molecular Weight: 99,633 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL
• NCBI Official Synonym Full Names: Cbl proto-oncogene, E3 ubiquitin protein ligase
• NCBI Official Symbol: CBL
• NCBI Official Synonym Symbols: CBL2; NSLL; C-CBL; RNF55; FRA11B
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL; oncogene CBL2; proto-oncogene c-Cbl; RING finger protein 55; signal transduction protein CBL; casitas B-lineage lymphoma proto-oncogene; fragile site, folic acid type, rare, fra(11)(q23.3); Cas-Br-M (murine) ecotropic retr
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL
• Protein Family: CBL-interacting protein kinase
• UniProt Gene Name: CBL
• UniProt Synonym Gene Names: CBL2; RNF55
• UniProt Entry Name: CBL_HUMAN

NCBI Description

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012]

Uniprot Description

CBL: an adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. Participates in signal transduction in hematopoietic cells.

Protein type: Ligase; EC 6.3.2.19; EC 6.3.2.-; Adaptor/scaffold; Oncoprotein; Motility/polarity/chemotaxis; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; plasma membrane; flotillin complex; nucleus; cytosol

Molecular Function: protein binding; signal transducer activity; ephrin receptor binding; zinc ion binding; phosphotyrosine binding; ubiquitin-protein ligase activity; calcium ion binding; SH3 domain binding; transcription factor activity; ligase activity

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; cell surface receptor linked signal transduction; fibroblast growth factor receptor signaling pathway; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; positive regulation of receptor-mediated endocytosis; negative regulation of apoptosis

Disease: Noonan Syndrome-like Disorder With Or Without Juvenile Myelomonocytic Leukemia