Rabbit anti-Human CBF Polyclonal Antibody | anti-CBF antibody

CBF, CT (CCAAT/Enhancer-binding Protein zeta, CCAAT-box-binding Transcription Factor, CCAAT-binding Factor, CBF, CEBPZ, CBF2) (AP)

Cat. Num. AAA6467785

• Gene Names: CEBPZ; CBF; CBF2; NOC1; HSP-CBF
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CBF; Polyclonal Antibody; CT (CCAAT/Enhancer-binding Protein zeta; CCAAT-box-binding Transcription Factor; CCAAT-binding Factor; CEBPZ; CBF2) (AP); anti-CBF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CEBPZ.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CBF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CEBPZ (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CEBPZ Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). CEBPZ(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CBF antibody

Antibodies; Transcription Factors

References

1. Uramoto, H., Biochem. J. 371(PT 2), 301-310 (2003). 2. Lum, L.S., Mol. Cell. Biol. 10(12), 6709-6717 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 42542392
• NCBI GeneID: 10153
• NCBI Accession #: NP_005751
• NCBI GenBank Nucleotide #: NM_005760
• UniProt Accession #: Q03701
• Molecular Weight: 121kDa
• NCBI Official Full Name: CCAAT/enhancer-binding protein zeta
• NCBI Official Synonym Full Names: CCAAT enhancer binding protein zeta
• NCBI Official Symbol: CEBPZ
• NCBI Official Synonym Symbols: CBF; CBF2; NOC1; HSP-CBF
• NCBI Protein Information: CCAAT/enhancer-binding protein zeta
• UniProt Protein Name: CCAAT/enhancer-binding protein zeta
• UniProt Gene Name: CEBPZ
• UniProt Synonym Gene Names: CBF2; CBF
• UniProt Entry Name: CEBPZ_HUMAN

NCBI Description

This gene belongs to the CEBP family. The encoded protein plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner. The protein is also involved in cell growth and differentiation, particularly, hematopoietic differentiation. Methylation of the promoter of this gene or mutations within the gene may be correlated with occurance of acute myeloid leukemia (AML). [provided by RefSeq, Jun 2016]

Uniprot Description

C/EBP-zeta: Stimulates transcription from the HSP70 promoter. Belongs to the CBF/MAK21 family.

Protein type: RNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2p22.2

Cellular Component: nucleus

Molecular Function: DNA binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter