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WB (Western Blot)
(FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)
Rabbit FGFR2 Polyclonal Antibody | anti-FGFR2 antibody
FGFR2 Antibody (N-term)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, mouse, rat, monkey
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FGFR2; Polyclonal Antibody; FGFR2 Antibody (N-term); Fibroblast growth factor receptor 2; FGFR-2; K-sam; KGFR; Keratinocyte growth factor receptor; CD332; BEK; KSAM; anti-FGFR2 antibody
Host
Rabbit
Reactivity
Human, mouse, rat, monkey
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human FGFR2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Positions
7-37
Sequence Length
709
Applicable Applications for anti-FGFR2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
IHC~~1:50~100
IF~~1:50~100
WB~~1:1000
IF~~1:50~100
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)
WB (Western Blot)
(FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)
WB (Western Blot)
(FGFR2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)
WB (Western Blot)
(FGFR2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from Hela, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. AAA28728 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. AAA28728 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, K562, T47D cell line (from left to right), using FGFR2 Antibody (R22). AAA28728 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, K562, T47D cell line (from left to right), using FGFR2 Antibody (R22). AAA28728 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term). AAA28728 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term). AAA28728 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with FGFR2 Antibody (N-term). AAA28728 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with FGFR2 Antibody (N-term). AAA28728 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). AAA28728 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). AAA28728 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-FGFR2 antibody
FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.
Product Categories/Family for anti-FGFR2 antibody
References
Freeman, K.W., et al., Cancer Res. 63(19):6237-6243 (2003). Goriely, A., et al., Science 301(5633):643-646 (2003). Fomenkov, A., et al., J. Biol. Chem. 278(26):23906-23914 (2003). Katoh, M., et al., Int. J. Mol. Med. 11(5):579-583 (2003). Katoh, M., et al., Int. J. Oncol. 22(5):1155-1159 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
92025
NCBI Official Full Name
fibroblast growth factor receptor 2 isoform 1
NCBI Official Synonym Full Names
fibroblast growth factor receptor 2
NCBI Official Symbol
FGFR2
NCBI Official Synonym Symbols
BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
NCBI Protein Information
fibroblast growth factor receptor 2
UniProt Protein Name
Fibroblast growth factor receptor 2
UniProt Gene Name
FGFR2
UniProt Synonym Gene Names
BEK; KGFR; KSAM; FGFR-2; KGFR
UniProt Entry Name
FGFR2_HUMAN
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Product Notes
The FGFR2 fgfr2 (Catalog #AAA28728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 7-37. The FGFR2 Antibody (N-term) reacts with Human, mouse, rat, monkey and may cross-react with other species as described in the data sheet. AAA Biotech's FGFR2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS). IHC~~1:50~100 IF~~1:50~100 WB~~1:1000. Researchers should empirically determine the suitability of the FGFR2 fgfr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGFR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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