Rabbit Cathepsin W Polyclonal Antibody | anti-CTSW antibody

Cathepsin W polyclonal antibody

Cat. Num. AAA3014969

• Gene Names: CTSW; LYPN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: Cathepsin W; Polyclonal Antibody; Cathepsin W polyclonal antibody; Lymphopain; CTSW; anti-CTSW antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Cathepsin W polyclonal antibody detects endogenous levels of Cathepsin W protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CTSW antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human Cathepsin W
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of Cathepsin W polyclonal antibody at 1:500 dilutionLaneA: RAW264.7 whole cell lysate)

Related Product Information for anti-CTSW antibody

Cathepsin W (lymphopain) and cathepsin F comprise a novel subgroup of cathepsin proteases, and are phylogenetically distinct from other human cathepsins. The cathepsin W gene maps to chromosome 11q13.1 and contains ten exons with introns ranging from 81-119 bp. Cathepsin W protein is expressed specifically in CD8+ T-lymphocyte. The expression of cathepsin W first occurs during the differentiation of thyrocytes to CD8+ T-lymphocytes, just as the thymocytes cease expression of CD4+ receptors. In transfected Cos-7 and Hela cells, cathepsin W localizes within the rough endoplasmic reticulum. Cathepsin W contains a unique 21-amino acid peptide insertion between the active site histidine and asparagine residues, in addition to a distictive 8-amino acid carboxy- terminal extension. An extended loop structure in the second or beta-sheet domain and an additional disulfide bind are two of several signature features of cathepsin W. Other features of cathepsin W include an additional cysteine, an S2 pocket and an additional residue. Cathepsin W may exist as a dimer with each monomer forming a disulfide bond.

NCBI and Uniprot Product Information

• NCBI GI #: 23110964
• NCBI GeneID: 1521
• NCBI Accession #: NP_001326
• NCBI GenBank Nucleotide #: NM_001335.3
• UniProt Accession #: P56202; Q86VT4
• Molecular Weight: 42 kDa (378aa)
• NCBI Official Full Name: cathepsin W preproprotein
• NCBI Official Synonym Full Names: cathepsin W
• NCBI Official Symbol: CTSW
• NCBI Official Synonym Symbols: LYPN
• NCBI Protein Information: cathepsin W
• UniProt Protein Name: Cathepsin W
• Protein Family: Cathepsin
• UniProt Gene Name: CTSW

NCBI Description

The protein encoded by this gene, a member of the peptidase C1 family, is a cysteine proteinase that may have a specific function in the mechanism or regulation of T-cell cytolytic activity. The encoded protein is found associated with the membrane inside the endoplasmic reticulum of natural killer and cytotoxic T-cells. Expression of this gene is up-regulated by interleukin-2. [provided by RefSeq, Jul 2008]

Uniprot Description

May have a specific function in the mechanism or regulation of T-cell cytolytic activity.