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Western Blot (WB) (Western blot analysis of Cathepsin L/V/K/H expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate; )

Rabbit Cathepsin L/V/K/H Polyclonal Antibody | anti-CTSL antibody

Anti-Cathepsin L/V/K/H Rabbit pAb

Gene Names
CTSL; MEP; CATL; CTSL1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
Cathepsin L/V/K/H; Polyclonal Antibody; Anti-Cathepsin L/V/K/H Rabbit pAb; Cathepsin; CTSL; CTSV; CTSK; CTSH; anti-CTSL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Cathepsin L/V/K/H Antibody detects endogenous levels of total Cathepsin L/V/K/H
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
333
Applicable Applications for anti-CTSL antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human Cathepsin L/V/K/H
Subcellular Location
Lysosome
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of Cathepsin L/V/K/H expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate; )

Western Blot (WB) (Western blot analysis of Cathepsin L/V/K/H expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate; )
Related Product Information for anti-CTSL antibody
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38kDa
NCBI Official Full Name
cathepsin L1 isoform 1 preproprotein
NCBI Official Synonym Full Names
cathepsin L
NCBI Official Symbol
CTSL
NCBI Official Synonym Symbols
MEP; CATL; CTSL1
NCBI Protein Information
cathepsin L1
UniProt Protein Name
Cathepsin L1
UniProt Gene Name
CTSL
UniProt Synonym Gene Names
CTSL1; MEP

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Important for the overall degradation of proteins in lysosomes.

Research Articles on CTSL

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Product Notes

The CTSL ctsl (Catalog #AAA4750781) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cathepsin L/V/K/H Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cathepsin L/V/K/H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 FC/FACS: 1:50. Researchers should empirically determine the suitability of the CTSL ctsl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cathepsin L/V/K/H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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