Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Hela Cells.)

Rabbit anti-Mouse Catalase (CAT) Polyclonal Antibody | anti-CAT antibody

Polyclonal Antibody to Catalase (CAT)

Gene Names
Cat; Cas1; Cs-1; Cas-1; 2210418N07
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Catalase (CAT); Polyclonal Antibody; Polyclonal Antibody to Catalase (CAT); anti-CAT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CAT. It has been selected for its ability to recognize CAT in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- G AFGYFEVTHD ITRYSKAKVF EHIGKRTPIA VRFSTVTGES GSADTVRDPR GFAVKFYTED GNWDLVGNNT PIFFIRDAIL FPSFIHSQKR NPQTHLKDPD MVWDFWSLRP ESLHQVSFLF SDRGIPDGHR HMNGYGSHTF KLVNADGEAV YCKFHYKTD
Sequence Length
527
Applicable Applications for anti-CAT antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CAT (Gly80~Asp239) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2065546
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Hela Cells.)

Western Blot (WB) (Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Hela Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant CAT, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant CAT, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Mouse Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P. Samples: Mouse Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,795 Da
NCBI Official Full Name
catalase
NCBI Official Synonym Full Names
catalase
NCBI Official Symbol
Cat
NCBI Official Synonym Symbols
Cas1; Cs-1; Cas-1; 2210418N07
NCBI Protein Information
catalase
UniProt Protein Name
Catalase
UniProt Gene Name
Cat
UniProt Synonym Gene Names
Cas-1; Cas1

Uniprot Description

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.

Protein type: Amino Acid Metabolism - tryptophan; Apoptosis; EC 1.11.1.6; Endoplasmic reticulum; Energy Metabolism - methane; Hydrolase; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 2 E2|2 54.43 cM

Cellular Component: cytosol; endoplasmic reticulum; extracellular exosome; extracellular space; focal adhesion; Golgi apparatus; intracellular membrane-bound organelle; lysosome; membrane; mitochondrial intermembrane space; mitochondrion; peroxisomal membrane; peroxisome; plasma membrane

Molecular Function: aminoacylase activity; antioxidant activity; catalase activity; enzyme binding; heme binding; metal ion binding; NADP binding; oxidoreductase activity; oxidoreductase activity, acting on peroxide as acceptor; peroxidase activity; protein homodimerization activity; receptor binding

Biological Process: aerobic respiration; cholesterol metabolic process; hemoglobin metabolic process; hydrogen peroxide catabolic process; kidney development; negative regulation of apoptosis; negative regulation of NF-kappaB transcription factor activity; osteoblast differentiation; positive regulation of cell division; positive regulation of NF-kappaB transcription factor activity; positive regulation of phosphoinositide 3-kinase cascade; protein homotetramerization; protein tetramerization; response to hydrogen peroxide; response to hypoxia; response to oxidative stress; response to reactive oxygen species; triacylglycerol metabolic process; ureteric bud development; UV protection

Research Articles on CAT

Similar Products

Product Notes

The CAT cat (Catalog #AAA2003879) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Catalase (CAT) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Catalase (CAT) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CAT cat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- G AFGYFEVTHD ITRYSKAKVF EHIGKRTPIA VRFSTVTGES GSADTVRDPR GFAVKFYTED GNWDLVGNNT PIFFIRDAIL FPSFIHSQKR NPQTHLKDPD MVWDFWSLRP ESLHQVSFLF SDRGIPDGHR HMNGYGSHTF KLVNADGEAV YCKFHYKTD. It is sometimes possible for the material contained within the vial of "Catalase (CAT), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.