Western Blot
(Western Blot analysis of Jurkat and MCF-7 cell lysates with anti-caspase 10 antibody)
Rabbit Caspase10 Polyclonal Antibody | anti-CASP-10 antibody
Caspase10 Antibody
Gene Names
CARD10; BIMP1; CARMA3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunofluorescence
Synonyms
Caspase10; Polyclonal Antibody; Caspase10 Antibody; anti-CASP-10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Form/Format
Lyophilized anti-caspase 10 antibody purified by peptide affinity column containing 1% BSA and 0.02% NaN3
Sequence Length
1032
Applicable Applications for anti-CASP-10 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Peptide ELISA: 1:5,000-1:20,000
WB: 1:200-500
IF: 1:50-100
Optimal dilutions/concentrations should be determined by the end user.
WB: 1:200-500
IF: 1:50-100
Optimal dilutions/concentrations should be determined by the end user.
Host Note
Rabbit polyclonal caspase 10 antibody was raised against a 15 amino acid peptide from near the C terminal residues of human caspase 10 protein.
Preparation and Storage
Dilution instruction and storage:
Already reconstituted in 200ul sterile H2O. The antibody is stable for at least one year when stored at -20ºC after reconstitution.
Avoid repeated freeze/defrost cycles.
Already reconstituted in 200ul sterile H2O. The antibody is stable for at least one year when stored at -20ºC after reconstitution.
Avoid repeated freeze/defrost cycles.
Western Blot
(Western Blot analysis of Jurkat and MCF-7 cell lysates with anti-caspase 10 antibody)
Western Blot
(Western Blot analysis of Jurkat and MCF-7 cell lysates with anti-caspase 10 antibody)
Immunofluorescence
(Immunofluorescent staining of HeLa cells showing Cytoplasmic staining with anti-Caspase 10 antibody at a dilution of 1/100)
Immunofluorescence
(Immunofluorescent staining of HeLa cells showing Cytoplasmic staining with anti-Caspase 10 antibody at a dilution of 1/100)
Related Product Information for anti-CASP-10 antibody
Rabbit polyclonal caspase 10 (1) antibody was raised against a 15 amino acid peptide from near the C terminal residues of human caspase 10 protein.
Product Categories/Family for anti-CASP-10 antibody
References
(1) Gerhard, D.S., Wagner, L., Feingold, E.A., et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14 (10B), 2121-2127 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
caspase recruitment domain-containing protein 10
NCBI Official Synonym Full Names
caspase recruitment domain family member 10
NCBI Official Symbol
CARD10
NCBI Official Synonym Symbols
BIMP1; CARMA3
NCBI Protein Information
caspase recruitment domain-containing protein 10
UniProt Protein Name
Caspase recruitment domain-containing protein 10
UniProt Gene Name
CARD10
UniProt Synonym Gene Names
CARMA3; Carma 3
NCBI Description
The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]
Uniprot Description
Activates NF-kappa-B via BCL10 and IKK.CautionSupposed to contain a SH3, a PDZ and a guanylate kinase-like domain. But none of these 3 domains are detected by PROSITE, Pfam or SMART.
Research Articles on CASP-10
Similar Products
Product Notes
The CASP-10 card10 (Catalog #AAA856639) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase10 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). Peptide ELISA: 1:5,000-1:20,000 WB: 1:200-500 IF: 1:50-100 Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the CASP-10 card10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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