Rabbit anti-Human SNAIL Polyclonal Antibody | anti-SNAIL antibody

SNAIL, NT (D24) (SNAI1, SNAH, Zinc finger protein SNAI1, Protein snail homolog 1) (MaxLight 750)

Cat. Num. AAA6349208

• Gene Names: SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: SNAIL; Polyclonal Antibody; NT (D24) (SNAI1; SNAH; Zinc finger protein SNAI1; Protein snail homolog 1) (MaxLight 750); SNAI1; Protein snail homolog 1; anti-SNAIL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SNAIL.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-SNAIL antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 9-39 in the N-terminal region of human SNAIL, UniProt Accession #O95863.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of SNAIL (arrow) in 293 cell lysates (2 ug/lane) using MBS648547 either nontransfected (Lane 1) or transiently transfected with the SNAIL gene (Lane 2).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human Spleen tissue reacted withMBS648547, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS648547 for immunohistochemistry.)

Western Blot (WB)

(HepG2 cells were incubated with HGF for the time periods indicated. LiCl and MG132 were added 8 h before lysis of the cells. Snail protein levels and beta actin as loading control were analyzed by WB.)

Product Categories/Family for anti-SNAIL antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Imai, T., et al., Am. J. Pathol. 163(4):1437-1447 (2003). 2. Yokoyama, K., et al., Int. J. Oncol. 22(4):891-898 (2003). 3. Guaita, S., et al., J. Biol. Chem. 277(42):39209-39216 (2002). 4. Blanco, M.J., et al., Oncogene 21(20):3241-3246 (2002). 5. Okubo, T., et al., Cancer Res. 61(4):1338-1346 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 18765741
• NCBI GeneID: 6615
• NCBI Accession #: NP_005976.2
• NCBI GenBank Nucleotide #: NM_005985.3
• UniProt Accession #: O95863; Q9P113; Q9UBP7; Q9UHH7; B2R842
• Molecular Weight: 29,083 Da
• NCBI Official Full Name: zinc finger protein SNAI1
• NCBI Official Synonym Full Names: snail family zinc finger 1
• NCBI Official Symbol: SNAI1
• NCBI Official Synonym Symbols: SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• NCBI Protein Information: zinc finger protein SNAI1; protein sna; snail 1 homolog; snail homolog 1; protein snail homolog 1; snail 1 zinc finger protein; snail 1, zinc finger protein
• UniProt Protein Name: Zinc finger protein SNAI1
• UniProt Gene Name: SNAI1
• UniProt Synonym Gene Names: SNAH; Protein sna
• UniProt Entry Name: SNAI1_HUMAN

NCBI Description

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

Snail1: a protein of the C2H2-type zinc-finger family that regulates transcription. Involved in embryonic mesoderm formation. Plays a role in the epithelial to mesenchymal transition (EMT). EMT is characterized by decreased levels of the epithelial markers E-cadherin and alpha- and beta-catenin, and increased cellular migration and expression of the mesenchymal markers fibronectin, vimentin, N-cadherin and smooth muscle alpha-actin.E Snail binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. NF-kappaB binds and regulates the snail promoter and is critical for EMT, and inhibition of NF-kappaB activity lowered snail levels and morphologically reversed the EMT.

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; kinase binding

Biological Process: osteoblast differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell migration; hair follicle morphogenesis; mesoderm formation; positive regulation of transcription, DNA-dependent; epithelial to mesenchymal transition; negative regulation of transcription from RNA polymerase II promoter; palate development; positive regulation of cell migration