Rabbit Caspase 9 Polyclonal Antibody | anti-Tyr153 antibody

Caspase 9 (Tyr153) Polyclonal Antibody

Cat. Num. AAA3000061

• Gene Names: CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Caspase 9; Polyclonal Antibody; Caspase 9 (Tyr153) Polyclonal Antibody; CASP 9; Caspase9; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10; CASP9; MCH6; anti-Tyr153 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caspase 9 (Tyr153) polyclonal antibody detects endogenous levels of full length caspase-9 (48 kDa) and large fragments of caspase-9 (35 kDa and 37kDa).
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 416

• Applicable Applications for anti-Tyr153 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 130-180 of Human Caspase 9.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Caspase 9 (Tyr153) polyclonal antibody at 1:500 dilutionLane1:LO2 whole cell lysate (40ug) Lane2:L02 treated with PBS (1×PBS,PH7. 4) for 30 minutes whole cell lysate (40ug) Lane3:L02 treated with PBS (1×PBS,PH7. 4) for 120 minutes whole cell lysate (40ug) Lane4:L02 treated with PBS (1×PBS,PH7. 4) for 240 minutes whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of Caspase 9 (Tyr153) pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:Hela treated with H2O2 (100nmol/ml, 25min, Na3O4V) whole cell lysate (40ug) Lane3:Hela treated with H2O2 (100nmol/ml, 25min, lambda-ppase) whole cell lysate (40ug))

Related Product Information for anti-Tyr153 antibody

Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6.

NCBI and Uniprot Product Information

• NCBI GI #: 14790124
• NCBI GeneID: 842
• NCBI Accession #: NP_001220.2
• NCBI GenBank Nucleotide #: NP_001220.2
• UniProt Accession #: P55211
• Molecular Weight: ~ 35, 37, 48kDa
• NCBI Official Full Name: caspase-9 isoform alpha
• NCBI Official Synonym Full Names: caspase 9
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• NCBI Protein Information: caspase-9
• UniProt Protein Name: Caspase-9
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6; CASP-9; APAF-3; ICE-LAP6
• UniProt Entry Name: CASP9_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.

Protein type: Apoptosis; EC 3.4.22.62; Protease

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: mitochondrion; nucleus; cytosol; apoptosome

Molecular Function: peptidase activity; protein binding; cysteine-type endopeptidase activity; enzyme activator activity; protein kinase binding; SH3 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; DNA damage response, signal transduction; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; response to antibiotic; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; response to cobalt ion; innate immune response; platelet formation; caspase activation via cytochrome c; response to DNA damage stimulus; aging