Rabbit Caspase 7, Cleavage Site Polyclonal Antibody | anti-CASP7 antibody

Caspase 7, Cleavage Site (Asp198) (Mch-3, ICE-LAP3, CMH-1)

Cat. Num. AAA610979

• Gene Names: CASP7; MCH3; CMH-1; ICE-LAP3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Caspase 7; Cleavage Site; Polyclonal Antibody; Cleavage Site (Asp198) (Mch-3; ICE-LAP3; CMH-1); Anti -Caspase 7; anti-CASP7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of the large fragment of caspase-7 following cleavage at (Asp198). Does not crossreact with full length caspase-7 or with other caspases. Species Crossreactivity: Human, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-CASP7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry.; Dilution: Western Blot: 1:1000. Detects a band at~20kD.; Immunoprecipitation: 1:100; Immunocytochemistry (ABC): 1:100
• Immunogen: Synthetic peptide corresponding to N-terminal residues adjacent to (Asp198) in human caspase-7 (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from Jurkat cells and 293 cells, untreated, cytochrome c-treated (0.25mg/ml, 1 hour) or etoposide-treated (25uM, 5 hours), using MBS610979.)

Western Blot (WB)

(Immunostaining of cerebellar neuron culture from newborn rat, untreated or tert-Butyl-hydroperoxide-treated (tBH, a free radical, 40uM for 30 minutes folowed by 3 hour recovery), showing colocalization of cleaved caspase-7 with apoptotic neurons, using MBS610979 (green) and propidium iodide (red).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using MBS610979 compared to a nonspecific negative control antibody (red).)

Related Product Information for anti-CASP7 antibody

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (2-4). Caspase-7 is an effector caspase (along with caspase-2 and -3), meaning that it cleaves essential cellular machinery rather than activating other caspases (5-8). Caspase-7 is cleaved by many enzymes, including caspases-3, -6, -8, -9 and granzyme B (1,4,5). Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase, or PARP (2,4).

Product Categories/Family for anti-CASP7 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 15718702
• NCBI GeneID: 840
• NCBI Accession #: NP_203126.1
• NCBI GenBank Nucleotide #: NM_033340.2
• UniProt Accession #: P55210; Q13364; Q53YD5; Q5SVL0; Q5SVL3; Q96BA0; B5BU45; D3DRB8; Q5SVL2
• Molecular Weight: 34,277 Da
• NCBI Official Full Name: caspase-7 isoform beta
• NCBI Official Synonym Full Names: caspase 7, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP7
• NCBI Official Synonym Symbols: MCH3; CMH-1; ICE-LAP3
• NCBI Protein Information: caspase-7; CASP-7; OTTHUMP00000020510; OTTHUMP00000020511; OTTHUMP00000020512; OTTHUMP00000020513; OTTHUMP00000020514; Lice2 alpha/beta/gamma; caspase 7 isoform delta; apoptotic protease MCH-3; ICE-like apoptotic protease 3; caspase 7, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-7
• Protein Family: Caspase
• UniProt Gene Name: CASP7
• UniProt Synonym Gene Names: MCH3
• UniProt Entry Name: CASP7_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq]

Uniprot Description

CASP7: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly- 217' bond. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Endoplasmic reticulum; Mitochondrial; EC 3.4.22.60; Apoptosis

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: apoptosis; caspase activation via cytochrome c; proteolysis; cell structure disassembly during apoptosis