Rabbit anti-Human Caspase 5 Polyclonal Antibody | anti-D121 antibody

Caspase 5 p20 (D121) Polyclonal Antibody

Cat. Num. AAA3008469

• Gene Names: CASP5; ICH-3; ICEREL-III; ICE(rel)III
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Caspase 5; Polyclonal Antibody; Caspase 5 p20 (D121) Polyclonal Antibody; Caspase-5; CASP-5; ICE(rel)-III; Protease ICH-3; Protease TY; Caspase-5 subunit p20; CASP5; ICH3; Caspase5; anti-D121 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caspase-5 p20 (D121) polyclonal antibody detects endogenous levels of the large subunit of active caspase-5 resulting from cleavage at Asp121.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 376

• Applicable Applications for anti-D121 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human Caspase-5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Caspase-5 p20 (D121) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate (40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate (40ug))

Related Product Information for anti-D121 antibody

Caspase-5 (also designated ICErel-III, TY, ICH-3 and caspase-12 in mouse), can cleave its own precursor, an activity that requires the cysteine 245 residue. Frameshift mutations in caspase-5 have been identified in MMP tumors of the endometrium, colon, and stomach, indicating the caspase-5 may be a new target gene in the microsatellite mutator pathway for cancer. The human caspase 5 gene maps to chromosome 11q22.2-q22.3 and encodes a protein whose expression is barely detectable in most tissues except brain, with highest expression levels being found in lung, liver and skeletal muscle.

NCBI and Uniprot Product Information

• NCBI GI #: 209870071
• NCBI GeneID: 838
• NCBI Accession #: NP_001129581.1
• NCBI GenBank Nucleotide #: NP_001129581.1
• UniProt Accession #: P51878
• Molecular Weight: ~ 20, 48kDa
• NCBI Official Full Name: caspase-5 isoform b
• NCBI Official Synonym Full Names: caspase 5
• NCBI Official Symbol: CASP5
• NCBI Official Synonym Symbols: ICH-3; ICEREL-III; ICE(rel)III
• NCBI Protein Information: caspase-5
• UniProt Protein Name: Caspase-5
• UniProt Gene Name: CASP5
• UniProt Synonym Gene Names: ICH3; CASP-5
• UniProt Entry Name: CASP5_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CASP5: Mediator of programmed cell death (apoptosis). Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits. Up-regulated by bacterial lipopolysaccharides (LPS). Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.58; Protease

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: neuron projection; cell soma; cytoplasm

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; substantia nigra development; apoptosis; regulation of inflammatory response; germ cell programmed cell death; positive regulation of interleukin-1 beta secretion; proteolysis