Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using CASP10 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human, mouse Caspase-10 Polyclonal Antibody | anti-CASP10 antibody
Caspase-10 Rabbit pab Antibody
Gene Names
CASP10; MCH4; ALPS2; FLICE2
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
Caspase-10; Polyclonal Antibody; Caspase-10 Rabbit pab Antibody; CASP10; ALPS2; FLICE2; MCH4; anti-CASP10 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
455
Applicable Applications for anti-CASP10 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Western Blot (WB): 1/500-1000
Immunohistochemistry (IHC): 1/50-100
Immunocytochemistry (ICC): 1/100
Immunoprecipitation (IP): 1/10
Flow Cytometry (FC/FACS): 1/50
Immunohistochemistry (IHC): 1/50-100
Immunocytochemistry (ICC): 1/100
Immunoprecipitation (IP): 1/10
Flow Cytometry (FC/FACS): 1/50
Immunogen
A synthetic -peptide of human Caspase-10
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using CASP10 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using CASP10 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-CASP10 antibody
Rabbit polyclonal to Human Caspase-10
Caspase-10 is a DED (death effector domain)-containing caspase and functions as an initiator caspase in Fas/TNF induced apoptosis (1). Four isoforms of caspase-10 have been identified: caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d. They have the same prodomain but different mature large and small subdomains (2-4). Upon death ligand-receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction (1-4). This complex processes procaspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, leading to apoptosis (3-6).
Caspase-10 is a DED (death effector domain)-containing caspase and functions as an initiator caspase in Fas/TNF induced apoptosis (1). Four isoforms of caspase-10 have been identified: caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d. They have the same prodomain but different mature large and small subdomains (2-4). Upon death ligand-receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction (1-4). This complex processes procaspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, leading to apoptosis (3-6).
References
1. Nunez, G. et al. (1998) Oncogene 17, 3237-3245. 2. Ng, P. W. et al. (1999) J. Biol. Chem. 274, 10301-10308. 3. Vincenz, C. and Dixit, V.M. (1997) J. Biol. Chem. 272, 6578-6583. 4. Fernandez-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 7464-7469. 5. Srinivasula, S. M. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 14486-14491. 6. Wang, J. et al. (1999) Cell 98, 47-58.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
521
NCBI Official Full Name
caspase-10 isoform 6 preproprotein
NCBI Official Synonym Full Names
caspase 10, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP10
NCBI Official Synonym Symbols
MCH4; ALPS2; FLICE2
NCBI Protein Information
caspase-10; CASP-10; FADD-like ICE2; apoptotic protease MCH-4; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; caspase 10, apoptosis-related cysteine protease; FAS-associated death domain protein interleukin-1B-converting enzyme 2
UniProt Protein Name
Caspase-10
Protein Family
UniProt Gene Name
CASP10
UniProt Synonym Gene Names
MCH4; CASP-10; FLICE2
UniProt Entry Name
CASPA_HUMAN
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Product Notes
The CASP10 casp10 (Catalog #AAA125680) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase-10 Rabbit pab Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Western Blot (WB): 1/500-1000 Immunohistochemistry (IHC): 1/50-100 Immunocytochemistry (ICC): 1/100 Immunoprecipitation (IP): 1/10 Flow Cytometry (FC/FACS): 1/50. Researchers should empirically determine the suitability of the CASP10 casp10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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