Rabbit anti-Human CASP9 Polyclonal Antibody | anti-CASP9 antibody

CASP9, ID (Caspase 9, Caspase-9, CASP-9, ICE-like Apoptotic Protease 6, ICE-LAP6, Apoptotic Protease Mch-6, Apoptotic Protease-activating Factor 3, APAF-3, MCH6) (MaxLight 650)

Cat. Num. AAA6467622

• Gene Names: CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CASP9; Polyclonal Antibody; ID (Caspase 9; Caspase-9; CASP-9; ICE-like Apoptotic Protease 6; ICE-LAP6; Apoptotic Protease Mch-6; Apoptotic Protease-activating Factor 3; APAF-3; MCH6) (MaxLight 650); anti-CASP9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CASP9.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-CASP9 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CASP9 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CASP9 Antibody (Center) western blot analysis in Hela cell line and mouse L929 tissue lysates (35ug/lane).This demonstrates the CASP9 antibody detected the CASP9 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP9 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CASP9 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-CASP9 antibody

Antibodies; Apoptosis; Caspase

References

1. Martin, M.C., et al., J. Biol. Chem. 280(15):15449-15455 (2005). 2. Raina, D., et al., J. Biol. Chem. 280(12):11147-11151 (2005). 3. Cornelis, S., et al., Oncogene 24(9):1552-1562 (2005). 4. Mohammad, R.M., et al., Mol. Cancer Ther. 4(1):13-21 (2005). 5. Tacconi, S., et al., Exp. Neurol. 190(1):254-262 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 14790124
• NCBI GeneID: 842
• NCBI Accession #: NP_001220
• NCBI GenBank Nucleotide #: NM_001229
• UniProt Accession #: P55211; O95348; Q53Y70; Q5JRU9; Q5UGI1; Q92852; Q9BQ62; Q9UEQ3; Q9UIJ8; B4E1A3
• Molecular Weight: 36,564 Da
• NCBI Official Full Name: caspase-9 isoform alpha
• NCBI Official Synonym Full Names: caspase 9, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• NCBI Protein Information: caspase-9; ICE-like apoptotic protease 6; apoptotic protease MCH-6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6; CASP-9; APAF-3; ICE-LAP6
• UniProt Entry Name: CASP9_HUMAN

Uniprot Description

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.

Protein type: Apoptosis; EC 3.4.22.62; Protease

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: mitochondrion; nucleus; cytosol; apoptosome

Molecular Function: peptidase activity; protein binding; cysteine-type endopeptidase activity; enzyme activator activity; protein kinase binding; SH3 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; DNA damage response, signal transduction; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; response to antibiotic; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; response to cobalt ion; innate immune response; platelet formation; caspase activation via cytochrome c; response to DNA damage stimulus; aging