Rabbit anti-Mouse CASP3 Polyclonal Antibody | anti-Casp3 antibody

mouse CASP3 Antibody (N-term S12)

Cat. Num. AAA9217101

• Gene Names: Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; A830040C14Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Synonyms: CASP3; Polyclonal Antibody; mouse CASP3 Antibody (N-term S12); Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; LICE; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12; Casp3; Cpp32; anti-Casp3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This mouse CASP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse CASP3.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 277

• Applicable Applications for anti-Casp3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:1000
• Subtitle: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Cytoplasm.
• Tissue Location: Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis.
• Antigen Region: 1-30 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-CASP3 Antibody at 1:1000 dilution + mouse spleen lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Casp3 antibody

Description: CASP3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.

Background: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.

Product Categories/Family for anti-Casp3 antibody

Primary Antibodies; Antibody Collections; Cancer; Cell Biology; Neuroscience; Amyotrophic Lateral Sclerosis (ALS)

References

Srikanth, C.V., et al. Science 330 (6002):390-393 (2010). Li, F., et al. Cell Stem Cell 7 (4):508-520 (2010). Wang, L., et al. J. Neurosci. 30 (39):13201-13210 (2010). Gascon, E., et al. J. Neurosci. 30 (37):12414-12423 (2010). Bohsali, A., et al. BMC Microbiol. 10, 237 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 6753284
• NCBI GeneID: 12367
• NCBI Accession #: NP_033940.1
• NCBI GenBank Nucleotide #: NM_009810.3
• UniProt Accession #: P70677; O08668; Q8CHV5; Q9QWI4
• Molecular Weight: 31475 Da
• NCBI Official Full Name: caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; A830040C14Rik
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_MOUSE

NCBI Description

This gene encodes a protein that belongs to a highly conserved family of cysteinyl aspartate-specific proteases that function as essential regulators of programmed cell death through apoptosis. Members of this family contain an N-terminal pro-domain and require cleavage at specific aspartate residues to become mature. The protein encoded by this gene belongs to a subgroup of cysteinyl aspartate-specific proteases that are activated by initiator caspases and that perform the proteolytic cleavage of apoptotic target proteins. Mice defective for this gene exhibit a variety of phenotypes including reduced neuronal apoptosis resulting in hyperplasias, hearing loss, attenuated osteogenic differentiation of bone marrow stromal stem cells, and pre- and post-natal lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Motility/polarity/chemotaxis; EC 3.4.22.56; Protease; Apoptosis

Cellular Component: cytoplasm; cytosol; lipid raft; nucleus

Molecular Function: aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; hydrolase activity; peptidase activity; phospholipase A2 activator activity; protease binding; protein binding; protein complex binding

Biological Process: apoptosis; B cell homeostasis; cell fate commitment; erythrocyte differentiation; heart development; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cell cycle; negative regulation of cyclin-dependent protein kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; positive regulation of apoptosis; positive regulation of catalytic activity; positive regulation of neuron apoptosis; protein processing; proteolysis; response to DNA damage stimulus; response to glucose stimulus; response to hydrogen peroxide; response to organic substance; response to UV; response to wounding; sensory perception of sound; T cell homeostasis