Sheep Casein Kinase 1, epsilon Polyclonal Antibody | anti-CSNK1E antibody

Casein Kinase 1, epsilon

Cat. Num. AAA622127

• Gene Names: CSNK1E; HCKIE; MGC10398
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Casein Kinase 1; epsilon; Polyclonal Antibody; Anti -Casein Kinase 1; anti-CSNK1E antibody

• Host: Sheep
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human, mouse and rat Casein Kinase 1. does not cross-react with rhCK1, rhCK1, or rhCK1
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-CSNK1E antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western blot: 1ug/ml
• Immunogen: E. coli-derived recombinant human CK1epsilon.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CSNK1E antibody

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms (a, 1, 2, 3, and) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 phosphorylates all three mammalian PER proteins, as well as BMAL1 and CRY, thereby modulating the activities of these circadian clock regulators.

Product Categories/Family for anti-CSNK1E antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 168985127
• NCBI GeneID: 1454
• UniProt Accession #: B0QY35; P49674; Q53EZ1; Q5U045
• Molecular Weight: 36,085 Da
• NCBI Official Full Name: casein kinase 1, epsilon
• NCBI Official Synonym Full Names: casein kinase 1, epsilon
• NCBI Official Symbol: CSNK1E
• NCBI Official Synonym Symbols: HCKIE; MGC10398
• NCBI Protein Information: casein kinase I isoform epsilon; CKIe; CKI-epsilon; OTTHUMP00000199211; OTTHUMP00000199212; OTTHUMP00000199213; OTTHUMP00000199217
• UniProt Protein Name: Casein kinase 1, epsilon
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1E
• UniProt Entry Name: B0QY35_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Uniprot Description

CK1E: an ubiquitous protein kinase of the CK1 family. Together with Dvl-1 and Frat-1 activate the Wnt signaling pathway. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. May play a role in cell cycle progression. Two splice variant isoforms have been described. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CK1-D and/or CK1-E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with SOCS3. Mutations in hamster and Drosophila orthologs have circadian rhythm phenotypes, and the circadian gene period (per) is a substrate in both human and fly. A coding SNP variant in human, which increases CK1 activity, is negatively associated with circadian disorder. LOF mutations and LOH seen in mammary ductal carcinoma.

Protein type: EC 2.7.11.1; Protein kinase, CK1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; organelle organization and biogenesis; endocytosis; signal transduction; DNA repair; regulation of circadian rhythm; protein amino acid phosphorylation; regulation of cell shape; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; circadian regulation of gene expression; G2/M transition of mitotic cell cycle