Rabbit anti-Human CASC5 Polyclonal Antibody | anti-CASC5 antibody
Phospho-CASC5 (Thr943/Thr1155) Antibody
Gene Names
CASC5; D40; CT29; KNL1; Spc7; hKNL-1; AF15Q14; PPP1R55; hSpc105
Reactivity
Human
Applications
ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
CASC5; Polyclonal Antibody; Phospho-CASC5 (Thr943/Thr1155) Antibody; ;Protein D40/AF15q14; AF15Q14; ALL1 fused gene from chromosome 15q14; Blinkin bub linking kinetochore protein; Cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CT29; D40; hKNL 1; hSpc105; KIAA1570; Kinetochore null 1 homolog; Kinetochore null protein 1; Kinetochore scaffold 1; KNL1; anti-CASC5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-CASC5 (Thr943/Thr1155) Antibody detects endogenous levels of CASC5 only when phosphorylated at Thr943/Thr1155.
Tissue Specificity: Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine, appendix and placenta, and in fetal liver and thymus.
Tissue Specificity: Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine, appendix and placenta, and in fetal liver and thymus.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CASC5 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human CASC5 around the phosphorylation site of Thr943/Thr1155.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Interacts with DSN1, MIS12, BUB1, BUB1B, NSL1 and ZWINT.
Subcellular Location
Nucleus. Chromosome>Centromere>Kinetochore.
Note: Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle.
Note: Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Related Product Information for anti-CASC5 antibody
Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
265,391 Da
NCBI Official Full Name
protein CASC5 isoform 2
NCBI Official Synonym Full Names
cancer susceptibility candidate 5
NCBI Official Symbol
CASC5
NCBI Official Synonym Symbols
D40; CT29; KNL1; Spc7; hKNL-1; AF15Q14; PPP1R55; hSpc105
NCBI Protein Information
protein CASC5; cancer/testis antigen 29; kinetochore null 1 homolog; kinetochore-null protein 1; blinkin, bub-linking kinetochore protein; protein phosphatase 1, regulatory subunit 55; ALL1-fused gene from chromosome 15q14 protein; cancer susceptibility c
UniProt Protein Name
Protein CASC5
Protein Family
UniProt Gene Name
CASC5
UniProt Synonym Gene Names
KIAA1570; KNL1; AF15q14; Blinkin; CT29
UniProt Entry Name
CASC5_HUMAN
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Product Notes
The CASC5 casc5 (Catalog #AAA9612635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CASC5 (Thr943/Thr1155) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CASC5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the CASC5 casc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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