Goat CARMA1 / CARD11 Polyclonal Antibody | anti-CARMA1 antibody

Goat Polyclonal to Human CARMA1 / CARD11

Cat. Num. AAA242607

• Gene Names: CARD11; PPBL; BENTA; BIMP3; IMD11; CARMA1
• Reactivity: Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rat; Predicted Reactivity: Bovine, Dog, Horse, Chicken (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CARMA1 / CARD11; Polyclonal Antibody; Goat Polyclonal to Human CARMA1 / CARD11; Anti-CARMA1 / CARD11 Antibody (C-Terminus) IHC-plus; CARD11; Card-maguk protein 1; CARMA1; Carma 1; BIMP3; Human CARMA1; anti-CARMA1 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rat; Predicted Reactivity: Bovine, Dog, Horse, Chicken (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CARD11.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1154

• Applicable Applications for anti-CARMA1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (2 ug/ml), WB (1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-QRKTIWVDEDQL from the C-terminus of human CARD11 (NP_115791.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster (100%); Elephant, Panda, Bovine, Dog, Horse, Turkey, Chicken (92%); Opossum (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: CARMA1 / CARD11 antibody was raised against synthetic peptide C-QRKTIWVDEDQL from the C-terminus of human CARD11 (NP_115791.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster (100%); Elephant, Panda, Bovine, Dog, Horse, Turkey, Chicken (92%); Opossum (83%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CARD11 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

Western Blot (WB)

(Antibody staining (1 ug/ml) of human Jurkat cells (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Related Product Information for anti-CARMA1 antibody

CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types.

Product Categories/Family for anti-CARMA1 antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 157743265
• NCBI GeneID: 84433
• NCBI Accession #: NP_115791.3
• NCBI GenBank Nucleotide #: NM_032415.5
• UniProt Accession #: Q9BXL7; Q2NKN7; Q548H3; A4D1Z7
• Molecular Weight: 133,284 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 11
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 11
• NCBI Official Symbol: CARD11
• NCBI Official Synonym Symbols: PPBL; BENTA; BIMP3; IMD11; CARMA1
• NCBI Protein Information: caspase recruitment domain-containing protein 11; carma 1; CARD-containing MAGUK protein 1; bcl10-interacting maguk protein 3
• UniProt Protein Name: Caspase recruitment domain-containing protein 11
• UniProt Gene Name: CARD11
• UniProt Synonym Gene Names: CARMA1; Carma 1
• UniProt Entry Name: CAR11_HUMAN

NCBI Description

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]

Uniprot Description

CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; immunological synapse; cytosol; lipid raft

Molecular Function: guanylate kinase activity; protein binding; CARD domain binding

Biological Process: positive regulation of cytokine production; thymic T cell selection; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; regulation of B cell differentiation; T cell costimulation; positive regulation of B cell proliferation; innate immune response; regulation of T cell differentiation; positive regulation of T cell proliferation; nucleotide phosphorylation

Disease: Persistent Polyclonal B-cell Lymphocytosis; Immunodeficiency 11; B-cell Expansion With Nfkb And T-cell Anergy