Rabbit anti-Human, Mouse CARMA1 Polyclonal Antibody | anti-CARD11 antibody

CARMA1 Antibody

Cat. Num. AAA151587

• Gene Names: CARD11; PPBL; BENTA; BIMP3; IMD11; CARMA1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: CARMA1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CARMA1; Polyclonal Antibody; CARMA1 Antibody; PPBL; BENTA; BIMP3; IMD11; Caspase recruitment domain-containing protein 11; CARD-containing MAGUK protein 1; Carma 1; caspase recruitment domain family; member 11; anti-CARD11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CARMA1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1154

• Applicable Applications for anti-CARD11 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 mug/mL. A band at approximately 125 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CARMA1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1.
• Buffer: CARMA1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CARMA1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CARMA1 expression in mouse thymus cell lysate with CARMA1 antibody at 0.5 (lane A), 1 (lane B), and 2 μg /ml (lane C), respectively. )

Immunohistochemistry (IHC)

(Immunohistochemistry of CARMA1 in mouse thymus with CARMA1 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CARMA1 in Mouse Spleen cells with CARMA1 antibody at 20 μg/mL.)

Related Product Information for anti-CARD11 antibody

CARMA1 Antibody: CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IκK complex, thereby regulating activation of the NF-κB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.

NCBI and Uniprot Product Information

• NCBI GI #: 172046231
• NCBI GeneID: 84433
• UniProt Accession #: Q9BXL7; Q2NKN7; Q548H3; A4D1Z7
• Molecular Weight: 133,284 Da
• NCBI Official Full Name: Caspase recruitment domain-containing protein 11
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 11
• NCBI Official Symbol: CARD11
• NCBI Official Synonym Symbols: PPBL; BENTA; BIMP3; IMD11; CARMA1
• NCBI Protein Information: caspase recruitment domain-containing protein 11; carma 1; CARD-containing MAGUK protein 1; bcl10-interacting maguk protein 3
• UniProt Protein Name: Caspase recruitment domain-containing protein 11
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD11
• UniProt Synonym Gene Names: CARMA1; Carma 1
• UniProt Entry Name: CAR11_HUMAN

NCBI Description

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]

Uniprot Description

CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; immunological synapse; cytosol; lipid raft

Molecular Function: guanylate kinase activity; protein binding; CARD domain binding

Biological Process: positive regulation of cytokine production; thymic T cell selection; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; regulation of B cell differentiation; T cell costimulation; positive regulation of B cell proliferation; innate immune response; regulation of T cell differentiation; positive regulation of T cell proliferation; nucleotide phosphorylation

Disease: Persistent Polyclonal B-cell Lymphocytosis; Immunodeficiency 11; B-cell Expansion With Nfkb And T-cell Anergy