Western Blot (WB)
(Western blot analysis of RPL37A expression in HeLa, HEK293T, Raw264.7, H9C2 whole cell lysates.)
Rabbit CARK Polyclonal Antibody | anti-CARK antibody
CARK Antibody
Gene Names
FPGT-TNNI3K; CARK; TNNI3K
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity chromatography
Synonyms
CARK; Polyclonal Antibody; CARK Antibody; Serine/threonine-protein kinase TNNI3K; Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinase; anti-CARK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of CARK protein.
Purity/Purification
Immunogen affinity chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/mL (varies by lot)
Sequence Length
856
Applicable Applications for anti-CARK antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CARK.
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of RPL37A expression in HeLa, HEK293T, Raw264.7, H9C2 whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RPL37A expression in HeLa, HEK293T, Raw264.7, H9C2 whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CARK staining in human heart formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CARK staining in human heart formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Product Categories/Family for anti-CARK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
104 kDa
NCBI Official Full Name
FPGT-TNNI3K fusion protein isoform b
NCBI Official Synonym Full Names
FPGT-TNNI3K readthrough
NCBI Official Symbol
FPGT-TNNI3K
NCBI Official Synonym Symbols
CARK; TNNI3K
NCBI Protein Information
FPGT-TNNI3K fusion protein
UniProt Protein Name
Serine/threonine-protein kinase TNNI3K
UniProt Gene Name
TNNI3K
NCBI Description
This locus represents naturally occurring read-through transcription from the neighboring fucose-1-phosphate guanylyltransferase (FPGT) and TNNI3 interacting kinase (TNNI3K) genes. Alternative splicing results in multiple transcript variants that are composed of in-frame exons from each individual gene. [provided by RefSeq, Dec 2010]
Uniprot Description
May play a role in cardiac physiology.
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Product Notes
The CARK tnni3k (Catalog #AAA9430892) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARK Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CARK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the CARK tnni3k for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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