Rabbit anti-Human, Mouse CA XII Polyclonal Antibody | anti-CAXII antibody

CA XII (N273) Polyclonal Antibody

Cat. Num. AAA3013909

• Gene Names: CA12; CAXII; CA-XII; T18816; HsT18816
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CA XII; Polyclonal Antibody; CA XII (N273) Polyclonal Antibody; Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3; CA12; anti-CAXII antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CA XII (N273) polyclonal antibody detects endogenous levels of CA XII protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 354

• Applicable Applications for anti-CAXII antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 243-303 of Human CA XII.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CA XII (N273) polyclonal antibody at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))

Related Product Information for anti-CAXII antibody

Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (alpha, beta, and gamma) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the alpha class. CA12, a member of the alpha class, is a plasma membrane protein with the catalytic domain in the extracellular space. CA12 is widely expressed in many tissues and is found at high levels in renal cell cancers. Its expression is induced by hypoxia in solid tumors and is considered to be a tumor marker. Genetic mutations in CA12 are associated with hyperchlorhidrosis.

NCBI and Uniprot Product Information

• NCBI GI #: 4502515
• NCBI GeneID: 771
• NCBI Accession #: NP_001209.1
• NCBI GenBank Nucleotide #: NP_001209.1
• UniProt Accession #: O43570
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: carbonic anhydrase 12 isoform 1
• NCBI Official Synonym Full Names: carbonic anhydrase 12
• NCBI Official Symbol: CA12
• NCBI Official Synonym Symbols: CAXII; CA-XII; T18816; HsT18816
• NCBI Protein Information: carbonic anhydrase 12
• UniProt Protein Name: Carbonic anhydrase 12
• UniProt Gene Name: CA12
• UniProt Synonym Gene Names: CA-XII
• UniProt Entry Name: CAH12_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

CA12: Reversible hydration of carbon dioxide. Homodimer. Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA), benzenesulfonamide and derivatives (4-carboxyethylbenzene-sulfonamide, 4- carboxyethylbenzene-sulfonamide ethyl ester, 4-(acetyl-2- aminoethyl)benzene-sulfonamide, 4-aminoethylbenzene-sulfonamide) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Energy Metabolism - nitrogen; EC 4.2.1.1; Membrane protein, integral; Lyase

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process; chloride ion homeostasis

Disease: Hyperchlorhidrosis, Isolated