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Western Blot (WB) (Western Blot: Sample: Rat Bone marrow lysate; Primary Ab: 5ug/ml Rabbit Anti-Human CPA3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Carboxypeptidase A3 Polyclonal Antibody | anti-CPA3 antibody

Polyclonal Antibody to Carboxypeptidase A3, Mast Cell (CPA3)

Gene Names
CPA3; MC-CPA
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Carboxypeptidase A3; Polyclonal Antibody; Polyclonal Antibody to Carboxypeptidase A3; Mast Cell (CPA3); anti-CPA3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CPA3. It has beenselected for its ability to recognize CPA3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-CPA3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CPA3 (Ile110~Ser417)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant CPA3 (Ile110~Ser417) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2065328
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Bone marrow lysate; Primary Ab: 5ug/ml Rabbit Anti-Human CPA3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Bone marrow lysate; Primary Ab: 5ug/ml Rabbit Anti-Human CPA3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,670 Da
NCBI Official Full Name
mast cell carboxypeptidase A preproprotein
NCBI Official Synonym Full Names
carboxypeptidase A3
NCBI Official Symbol
CPA3
NCBI Official Synonym Symbols
MC-CPA
NCBI Protein Information
mast cell carboxypeptidase A
UniProt Protein Name
Mast cell carboxypeptidase A
UniProt Gene Name
CPA3
UniProt Synonym Gene Names
MC-CPA

NCBI Description

This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein is proteolytically processed to generate a mature protease that is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Expression of this gene may be elevated in human asthma patients. [provided by RefSeq, Nov 2015]

Uniprot Description

CPA3: Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase. [provided by RefSeq, Jan 2009]

Protein type: EC 3.4.17.1; Protease

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: extracellular matrix; extracellular region; extracellular space; secretory granule; transport vesicle

Molecular Function: metallocarboxypeptidase activity; zinc ion binding

Biological Process: angiotensin maturation; proteolysis

Research Articles on CPA3

Similar Products

Product Notes

The CPA3 cpa3 (Catalog #AAA2027211) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Carboxypeptidase A3, Mast Cell (CPA3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Carboxypeptidase A3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CPA3 cpa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carboxypeptidase A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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