Goat anti-Human Carbohydrate Sulfotransferase 2 Polyclonal Antibody | anti-CHST2 antibody

Carbohydrate Sulfotransferase 2 (CHST2, Carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2, GlcNAc6ST-1, Gn6ST, GN6ST, GST2, GST-2, N-acetylglucosamine 6-O

Cat. Num. AAA620338

• Gene Names: CHST2; C6ST; GST2; GST-2; Gn6ST-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Carbohydrate Sulfotransferase 2; Polyclonal Antibody; Carbohydrate Sulfotransferase 2 (CHST2; Carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2; C6ST; Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GlcNAc6ST-1; Gn6ST; GN6ST; GST2; GST-2; N-acetylglucosamine 6-O; Anti -Carbohydrate Sulfotransferase 2 (CHST2; anti-CHST2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHST2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

• Applicable Applications for anti-CHST2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunoprecipitation.; Dilution: ELISA: 0.5-1.0ug/ml; Western Blot: 0.1-0.2ug/ml
• Immunogen: Human CHST2 aa76- 530.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CHST2 antibody

The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also

Product Categories/Family for anti-CHST2 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 27369497
• NCBI GeneID: 9435
• NCBI Accession #: NP_004258.2
• NCBI GenBank Nucleotide #: NM_004267.3
• UniProt Accession #: Q9Y4C5; Q2M370; Q9GZN5; Q9UED5; Q9Y6F2; D3DNG5
• Molecular Weight: 57,857 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 2
• NCBI Official Synonym Full Names: carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2
• NCBI Official Symbol: CHST2
• NCBI Official Synonym Symbols: C6ST; GST2; GST-2; Gn6ST-1
• NCBI Protein Information: carbohydrate sulfotransferase 2; glcNAc6ST-1; OTTHUMP00000215002; N-acetylglucosamine 6-O-sulfotransferase 1; carbohydrate (chondroitin 6/keratan) sulfotransferase 2; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2
• UniProt Protein Name: Carbohydrate sulfotransferase 2
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST2
• UniProt Synonym Gene Names: GN6ST
• UniProt Entry Name: CHST2_HUMAN

NCBI Description

N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).[supplied by OMIM]

Uniprot Description

CHST2: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 2.8.2.-; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: Golgi membrane; intrinsic to Golgi membrane; integral to membrane; trans-Golgi network

Molecular Function: sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; multicellular organismal development; keratan sulfate biosynthetic process; N-acetylglucosamine metabolic process; carbohydrate metabolic process; pathogenesis; inflammatory response