Rabbit CARABIN Polyclonal Antibody | anti-TBC1D10C antibody

RABBIT ANTI CARABIN (N-TERMINAL)

Cat. Num. AAA222937

• Gene Names: TBC1D10C; EPI64C; CARABIN
• Reactivity: Mouse; ; N.B. Antibody reactivity and working conditions may vary between species.
• Applications: Immunohistochemistry, Western Blot
• Synonyms: CARABIN; Polyclonal Antibody; RABBIT ANTI CARABIN (N-TERMINAL); anti-TBC1D10C antibody

• Host: Rabbit
• Reactivity: Mouse; ; N.B. Antibody reactivity and working conditions may vary between species.
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises human Carabin, also known as TBC1 domain family member 10C (TBC1D10C), a 50 kDa protein belonging to the TBC1 domain family of proteins. ; ; Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Studies demonstrate that Carabin is part of a negative regulatory loop for the intracellular TCR signalling pathway and acts as an inhibitor of the Ras signalling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras. ; ; Carabin is most abundantly expressed in spleen and peripheral blood leucocytes. ; ; This item does not cross react with either TBC1D10A or TBC1D10B.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 310

• Applicable Applications for anti-TBC1D10C antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 55kDa in human spleen tissue cell lysates.; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: A 17 amino acid peptide from near the amino terminus of human Carabin.
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antiserum to human Carabin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from Daudi, Burkitt's lymphoma derived cells probed with Rabbit anti Human Carabin (MBS222937) at 1(A) and 2(B) ug/ml)

Testing Data #2

(Formalin fixed, paraffin embedded human spleen with Rabbit anti Human Carabin (MBS222937) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-TBC1D10C antibody

MBS222937 recognises human Carabin, also known as TBC1 domain family member 10C (TBC1D10C), a 50 kDa protein belonging to the TBC1 domain family of proteins. Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Studies demonstrate that Carabin is part of a negative regulatory loop for the intracellular TCR signalling pathway and acts as an inhibitor of the Ras signalling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras. Carabin is most abundantly expressed in spleen and peripheral blood leucocytes. MBS222937 does not cross react with either TBC1D10A or TBC1D10B.

NCBI and Uniprot Product Information

• NCBI GI #: 374671768
• NCBI GeneID: 374403
• NCBI Accession #: NP_001243437.1
• NCBI GenBank Nucleotide #: NM_001256508.1
• UniProt Accession #: Q8IV04; G3V1D6
• Molecular Weight: 32,887 Da
• NCBI Official Full Name: carabin isoform 2
• NCBI Official Synonym Full Names: TBC1 domain family, member 10C
• NCBI Official Symbol: TBC1D10C
• NCBI Official Synonym Symbols: EPI64C; CARABIN
• NCBI Protein Information: carabin
• UniProt Protein Name: Carabin
• Protein Family: Carabin
• UniProt Gene Name: TBC1D10C
• UniProt Entry Name: TB10C_HUMAN

NCBI Description

The protein encoded by this gene has an N-terminal Rab-GTPase domain and a binding site at the C-terminus for calcineurin, and is an inhibitor of both the Ras signaling pathway and calcineurin, a phosphatase regulated by calcium and calmodulin. Genes encoding similar proteins are located on chromosomes 16 and 22. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

TBC1D10C: Inhibits the Ras signaling pathway through its intrinsic Ras GTPase-activating protein (GAP) activity. Acts as a negative feedback inhibitor of the calcineurin signaling pathway that also mediates crosstalk between calcineurin and Ras.

Protein type: GAPs; GAPs, Rab

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: filopodium membrane; membrane; plasma membrane

Biological Process: negative regulation of B cell activation; negative regulation of NFAT protein import into nucleus; retrograde transport, endosome to Golgi