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Western Blot (WB) (Western Blot analysis of CAPZA2 polyclonal antibody)

Rabbit anti-Human, Mouse CAPZA2 Polyclonal Antibody | anti-CAPZA2 antibody

CAPZA2 Polyclonal Antibody

Gene Names
CAPZA2; CAPZ; CAPPA2
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CAPZA2; Polyclonal Antibody; CAPZA2 Polyclonal Antibody; CAPPA2; Capping protein (actin filament) muscle Z line; alpha 2; CapZ alpha 2; CapZ alpha-2; CAPZ; CAZA2; F actin capping protein subunit alpha 2; F-actin-capping protein subunit alpha-2; anti-CAPZA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CAPZA2 polyclonal antibody detects endogenous levels of CAPZA2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
286
Applicable Applications for anti-CAPZA2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
Immunogen
Recombinant full length Human CAPZA2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of CAPZA2 polyclonal antibody)

Western Blot (WB) (Western Blot analysis of CAPZA2 polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of HepG2 cells, using CAPZA2 polyclonal antibody)

Immunofluorescence (IF) (Immunofluorescence analysis of HepG2 cells, using CAPZA2 polyclonal antibody)
Related Product Information for anti-CAPZA2 antibody
The F-Actin family of capping proteins includes CapZ-alpha1, CapZ-alpha2, CapZ-alpha3 and CapZ-beta3, all of which function in a calcium-dependent manner and bind to the fast growing barbed end of Actin filaments, thereby blocking protein exchange at these ends. The F-Actin capping protein complex is a heterodimer consisting of alpha and beta subunits that caps the barbed ends of Actin filaments and nucleates the polymerization of Actin monomers, yet does not sever Actin filaments.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
830
UniProt Accession #
Molecular Weight
~ 38kDa
NCBI Official Full Name
F-actin-capping protein subunit alpha-2
NCBI Official Synonym Full Names
capping actin protein of muscle Z-line subunit alpha 2
NCBI Official Symbol
CAPZA2
NCBI Official Synonym Symbols
CAPZ; CAPPA2
NCBI Protein Information
F-actin-capping protein subunit alpha-2
UniProt Protein Name
F-actin-capping protein subunit alpha-2
Protein Family
UniProt Gene Name
CAPZA2
UniProt Entry Name
CAZA2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the F-actin capping protein alpha subunit family. It is the alpha subunit of the barbed-end actin binding protein Cap Z. By capping the barbed end of actin filaments, Cap Z regulates the growth of the actin filaments at the barbed end. [provided by RefSeq, Jul 2008]

Uniprot Description

CAPZA2: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Belongs to the F-actin-capping protein alpha subunit family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 7q31.2-q31.3

Cellular Component: cortical cytoskeleton; F-actin capping protein complex; membrane; extracellular region; cytosol; actin cytoskeleton

Molecular Function: actin binding

Biological Process: innate immune response; protein complex assembly; cell motility; barbed-end actin filament capping; blood coagulation

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Product Notes

The CAPZA2 capza2 (Catalog #AAA3008025) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CAPZA2 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CAPZA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the CAPZA2 capza2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPZA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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