Western Blot (WB)
(Western Blot analysis of CAPN1 expression in transfected 293T cell line by CAPN1 polyclonal antibody. Lane 1: CAPN1 transfected lysate (81.9kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human CAPN1 Polyclonal Antibody | anti-CAPN1 antibody
CAPN1 (Calpain-1 Catalytic Subunit, Calcium-activated Neutral Proteinase 1, CANP 1, Calpain mu-type, Calpain-1 Large Subunit, Cell Proliferation-inducing Gene 30 Protein, Micromolar-calpain, muCANP, CANPL1, PIG30) (Biotin)
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CAPN1; Polyclonal Antibody; CAPN1 (Calpain-1 Catalytic Subunit; Calcium-activated Neutral Proteinase 1; CANP 1; Calpain mu-type; Calpain-1 Large Subunit; Cell Proliferation-inducing Gene 30 Protein; Micromolar-calpain; muCANP; CANPL1; PIG30) (Biotin); anti-CAPN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CAPN1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-CAPN1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human CAPN1, aa1-714 (NP_005177.2).
Immunogen Sequence
MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of CAPN1 expression in transfected 293T cell line by CAPN1 polyclonal antibody. Lane 1: CAPN1 transfected lysate (81.9kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of CAPN1 expression in transfected 293T cell line by CAPN1 polyclonal antibody. Lane 1: CAPN1 transfected lysate (81.9kD). Lane 2: Non-transfected lysate.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CAPN1 and F2R. HeLa cells were stained with CAPN1 rabbit purified polyclonal 1:1200 and F2R mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CAPN1 and F2R. HeLa cells were stained with CAPN1 rabbit purified polyclonal 1:1200 and F2R mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)
Related Product Information for anti-CAPN1 antibody
Calpain 1 (mu-calpain), a calcium-dependent cysteine C2 endopeptidase, is involved in myofibrillar protein degradation. In skeletal muscles, activation of Calpain I results in degradation of skeletal structural proteins, improving meat tenderness. Calpains I is inhibited by the endogenous inhibitor calpastatin and by leupeptin. Calpastatin is inactivated in postmortem skeletal muscle. Activity and expression of calpains in peripheral lymphoid organs may play an important role in T cell migration.
Product Categories/Family for anti-CAPN1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81,890 Da
NCBI Official Full Name
calpain-1 catalytic subunit
NCBI Official Synonym Full Names
calpain 1
NCBI Official Symbol
CAPN1
NCBI Official Synonym Symbols
CANP; muCL; CANP1; SPG76; CANPL1; muCANP
NCBI Protein Information
calpain-1 catalytic subunit
Protein Family
NCBI Description
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Research Articles on CAPN1
Similar Products
Product Notes
The CAPN1 (Catalog #AAA6372170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CAPN1 (Calpain-1 Catalytic Subunit, Calcium-activated Neutral Proteinase 1, CANP 1, Calpain mu-type, Calpain-1 Large Subunit, Cell Proliferation-inducing Gene 30 Protein, Micromolar-calpain, muCANP, CANPL1, PIG30) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAPN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CAPN1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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