Rabbit CAND1 Polyclonal Antibody | anti-CAND1 antibody

Anti-CAND1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100765

• Gene Names: CAND1; TIP120; TIP120A
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: CAND1; Polyclonal Antibody; Anti-CAND1 Antibody; Rabbit Polyclonal; CAND1/TIP120A Antibody; Rabbit PAb; Antigen Affinity Purified; cullin-associated and neddylation-dissociated 1; Anti-TIP120 Antibody; Anti-TIP120A Antibody; anti-CAND1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CAND1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CAND1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:500-1:2000; ICC/IF: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human CAND1/TIP120A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human CAND1/TIP120A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining CAND1 in human ovarian cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining CAND1 in mouse kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining CAND1 in rat kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of CAND1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- CAND1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to cytoplasm and nucleus.)

Related Product Information for anti-CAND1 antibody

Cullin-associated NEDD8-dissociated 1 (CAND1) plays a vital role in regulating the activity of Cullin-RING ubiquitin ligases (CRLs), which are frequently dysregulated in cancer. CAND1 expression as a predictor of poor prognosis for HCC patients. CAND1 silencing suppressed HCC cell proliferation by inducing caspase-8/RIP1-dependent apoptosis. That CAND1 could be a new therapeutic target for liver cancer. The miR-33a was decreased in non-small cell lung cancer (NSCLC) tissues compared with the para-carcinoma tissues, whereas its target mRNA of cullin-associated NEDD8-dissociated protein 1 (CAND1) was increased in NSCLC tissues. the miR-33a and CAND1 played an important role in lung cancer proliferation and cell migration. CAND1 inhibits the ubiquitylation of rabphilin-3A and positively regulates AVP secretion. CAND1 is a regulator of hormone or neurotransmitter secretion. The Cullin exchange factor CAND1 is a biochemical target of C60. We also identified CAND1 in an shRNA library screen for EBV lytic reactivation.

NCBI and Uniprot Product Information

• NCBI GI #: 67460541
• NCBI GeneID: 55832
• UniProt Accession #: Q86VP6; O94918; Q6PIY4; Q8NDJ4; Q96JZ9; Q96T19; Q9BTC4; Q9H0G2; Q9P0H7; Q9UF85; B2RAU3
• Molecular Weight: 136 kDa
• NCBI Official Full Name: Cullin-associated NEDD8-dissociated protein 1
• NCBI Official Synonym Full Names: cullin associated and neddylation dissociated 1
• NCBI Official Symbol: CAND1
• NCBI Official Synonym Symbols: TIP120; TIP120A
• NCBI Protein Information: cullin-associated NEDD8-dissociated protein 1
• UniProt Protein Name: Cullin-associated NEDD8-dissociated protein 1
• UniProt Gene Name: CAND1
• UniProt Synonym Gene Names: KIAA0829; TIP120; TIP120A; TBP-interacting protein 120A

NCBI Description

This gene encodes an essential regulator of Cullin-RING ubiquitin ligases, which are in involved in ubiquitinylation of proteins degraded by the Ub proteasome system. The encoded protein binds to unneddylated cullin-RING box protein complexes and acts as an inhibitor of cullin neddylation and of Skp1, cullin, and F box ubiquitin ligase complex assembly and activity. In mammalian cell culture, this protein predominantly localizes to the cytoplasm. Knockdown of this gene in preadipocytes results in blocked adipogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]

Uniprot Description

Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.