Western Blot (WB)
(Anti-CAMKK2 antibody, MBS175432, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Brain Tissue Lysate )
Rabbit CAMKK2 Polyclonal Antibody | anti-CAMKK2 antibody
Anti-CAMKK2 antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration: 0.1-0.5 ug/mL
Tested Species: Human, Mouse, Rat
Immunohistochemistry-Paraffin
Concentration: 0.5-1 ug/mL
Tested Species: Mouse
Cloncluded Species: Human, Rat
Antigen Retrieval: By Heat
Other applications have not been tested.
Optimal dilutions should be detrmined by end user.
Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-CAMKK2 antibody, MBS175432, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Brain Tissue Lysate )
Western Blot (WB)
(Anti-CAMKK2 antibody, MBS175432, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Brain Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-CAMKK2 antibody, MBS175432, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-CAMKK2 antibody, MBS175432, IHC(P)IHC(P): Rat Brain Tissue )
Background: CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, is an enzyme that in humans is encoded by the CAMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent(CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. CaMKK2 regulates production of the appetite stimulating hormone neuropeptide Y and functions as an AMPK kinase in the hypothalamus. It also has an important role in the development of hyperalgesia and tolerance to opioid analgesic drugs, through reduction in downstream signalling pathways and mu opioid receptor downregulation. Inhibition of CaMKK2 in mice reduces appetite and promotes weight loss.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq, Jul 2012]
Uniprot Description
CAMKK2: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin- binding domain are inactive. Seems to be involved in hippocampal activation of CREB1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy; EC 2.7.11.17; Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; CAMKK family; Meta subfamily
Chromosomal Location of Human Ortholog: 12q24.2
Cellular Component: cell projection; cytoplasm; intracellular; nucleus
Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; protein-tyrosine kinase activity; calcium ion binding; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; calcium-mediated signaling; MAPKKK cascade; regulation of protein kinase activity; protein amino acid phosphorylation
Research Articles on CAMKK2
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Product Notes
The CAMKK2 camkk2 (Catalog #AAA175432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CAMKK2 antibody reacts with Human, Mouse, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's CAMKK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot Concentration: 0.1-0.5 ug/mL Tested Species: Human, Mouse, Rat Immunohistochemistry-Paraffin Concentration: 0.5-1 ug/mL Tested Species: Mouse Cloncluded Species: Human, Rat Antigen Retrieval: By Heat Other applications have not been tested.Optimal dilutions should be detrmined by end user. Researchers should empirically determine the suitability of the CAMKK2 camkk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAMKK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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