Mucin-1 Recombinant Protein | MUC1 recombinant protein

Recombinant Human Mucin-1

Cat. Num. AAA969768

• Gene Names: MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Mucin-1; Recombinant Human Mucin-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227; MUC1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 24-273
• Sequence: SGHASSTPGGEKETSATQRSSVPSSTEKNALSTGVSFFFLSFHISNLQFNSSLEDPSTDYYQELQRDISEMFLQIYKQGGFLGLSNIKFRPGSVVVQLTLAFREGTINVHDVETQFNQYKTEAASRYNLTISDVSVSDVPFPFSAQSGAGVPGWGIALLVLVCVLVALAIVYLIALAVCQCRRKNYGQLDIFPARDTYHPMSEYPTYHTHGRYVPPSSTDRSPYEKVSAGNGGSSLSYTNPAVAATSANL
• Sequence Length: 264

• Product Note: Applications are user defined. Product is developed and quality control tested in house, data or additional information may be provided upon request. The researcher needs to establish and confirm the suitability of the product for their application.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

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Related Product Information for MUC1 recombinant protein

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Product Categories/Family for MUC1 recombinant protein

Signal Transduction; Raw Materials Assay Development

References

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Differential splicing may generate multiple protein forms.Wreschner D.H., Hareuveni M., Tsarfaty I., Smorodinsky N., Horev J., Zaretsky J., Kotkes P., Weiss M., Lathe R., Dion A., Keydar I.Eur. J. Biochem. 189:463-473(1990) A transcribed gene, containing a variable number of tandem repeats, codes for a human epithelial tumor antigen. cDNA cloning, expression of the transfected gene and over-expression in breast cancer tissue.Hareuveni M., Tsarfaty I., Zaretsky J., Kotkes P., Horev J., Zrihan S., Weiss M., Green S., Lathe R., Keydar I., Wreschner D.H.Eur. J. 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A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats.Gendler S.J., Taylor-Papadimitriou J., Duhig T., Rothbard J., Burchell J.J. Biol. Chem. 263:12820-12823(1988) Sequence analysis of the 5' region of the human DF3 breast carcinoma-associated antigen gene.Abe M., Siddiqui J., Kufe D.Biochem. Biophys. Res. Commun. 165:644-649(1989) Preoperative diagnosis of thyroid papillary carcinoma by reverse transcriptase polymerase chain reaction of the MUC1 gene.Weiss M., Baruch A., Keydar I., Wreschner D.H.3.0.CO;2-A>Int. J. Cancer 66:55-59(1996) Mucin mRNA expression in lung adenocarcinoma cell lines and tissues.Yu C.J., Yang P.C., Shew J.Y., Hong T.M., Yang S.C., Lee Y.C., Lee L.N., Luh K.T., Wu C.W.Oncology 53:118-126(1996) Buluwela L., Liu Q., Luqmani Y.A., Gomm J.J., Coombes R.C. Identification of MUC1 proteolytic cleavage sites in vivo.Parry S., Silverman H.S., McDermott K., Willis A., Hollingsworth M.A., Harris A.Biochem. Biophys. Res. Commun. 283:715-720(2001) The MUC1 SEA module is a self-cleaving domain.Levitin F., Stern O., Weiss M., Gil-Henn C., Ziv R., Prokocimer Z., Smorodinsky N.I., Rubinstein D.B., Wreschner D.H.J. Biol. Chem. 280:33374-33386(2005) High density O-glycosylation on tandem repeat peptide from secretory MUC1 of T47D breast cancer cells.Mueller S., Alving K., Peter-Katalinic J., Zachara N., Gooley A.A., Hanisch F.-G.J. Biol. Chem. 274:18165-18172(1999) Tyrosine phosphorylation of the MUC1 breast cancer membrane proteins. Cytokine receptor-like molecules.Zrihan-Licht S., Baruch A., Elroy-Stein O., Keydar I., Wreschner D.H.FEBS Lett. 356:130-136(1994) Association of the DF3/MUC1 breast cancer antigen with Grb2 and the Sos/Ras exchange protein.Pandey P., Kharbanda S., Kufe D.Cancer Res. 55:4000-4003(1995) Studies on the order and site specificity of GalNAc transfer to MUC1 tandem repeats by UDP-GalNAc polypeptide N-acetylgalactosaminyltransferase from milk or mammary carcinoma cells.Stadie T.R., Chai W., Lawson A.M., Byfield P.G., Hanisch F.G.Eur. J. Biochem. 229:140-147(1995) Interaction of the DF3/MUC1 breast carcinoma-associated antigen and beta-catenin in cell adhesion.Yamamoto M., Bharti A., Li Y., Kufe D.J. Biol. Chem. 272:12492-12494(1997) Localization of O-glycosylation sites on glycopeptide fragments from lactation-associated MUC1. All putative sites within the tandem repeat are glycosylation targets in vivo.Mueller S., Goletz S., Packer N.H., Gooley A.A., Lawson A.M., Hanisch F.-G.J. Biol. Chem. 272:24780-24793(1997) Developmental expression of mucin genes in the human gastrointestinal system.Reid C.J., Harris A.Gut 42:220-226(1998) Localization of O-glycosylation sites of MUC1 tandem repeats by QTOF ESI mass spectrometry.Hanisch F.G., Green B.N., Bateman R., Peter-Katalinic J.3.3.CO;2-V>J. Mass Spectrom. 33:358-362(1998) Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin.Li Y., Bharti A., Chen D., Gong J., Kufe D.Mol. Cell. Biol. 18:7216-7224(1998) The breast cancer-associated MUC1 gene generates both a receptor and its cognate binding protein.Baruch A., Hartmann M.-L., Yoeli M., Adereth Y., Greenstein S., Stadler Y., Skornik Y., Zaretsky J., Smorodinsky N.I., Keydar I., Wreschner D.H.Cancer Res. 59:1552-1561(1999) MUC1 mucin core protein binds to the domain 1 of ICAM-1.Hayashi T., Takahashi T., Motoya S., Ishida T., Itoh F., Adachi M., Hinoda Y., Imai K.Digestion 63 Suppl. 1:87-92(2001) Mucin MUC1 is seen in cell surface protrusions together with ezrin in immunoelectron tomography and is concentrated at tips of filopodial protrusions in MCF-7 breast carcinoma cells.Bennett R. Jr., Jaervelae T., Engelhardt P., Kostamovaara L., Sparks P., Carpen O., Turunen O., Vaheri A.J. Histochem. Cytochem. 49:67-77(2001) The c-Src tyrosine kinase regulates signaling of the human DF3/MUC1 carcinoma-associated antigen with GSK3 beta and beta-catenin.Li Y., Kuwahara H., Ren J., Wen G., Kufe D.J. Biol. Chem. 276:6061-6064(2001) Identification and topology of variant sequences within individual repeat domains of the human epithelial tumor mucin MUC1.Engelmann K., Baldus S.E., Hanisch F.-G.J. Biol. Chem. 276:27764-27769(2001) The epidermal growth factor receptor regulates interaction of the human DF3/MUC1 carcinoma antigen with c-Src and beta-catenin.Li Y., Ren J., Yu W., Li Q., Kuwahara H., Yin L., Carraway K.L. III, Kufe D.J. Biol. Chem. 276:35239-35242(2001) Protein kinase C delta regulates function of the DF3/MUC1 carcinoma antigen in beta-catenin signaling.Ren J., Li Y., Kufe D.J. Biol. Chem. 277:17616-17622(2002) Identification of four sites of stimulated tyrosine phosphorylation in the MUC1 cytoplasmic tail.Wang H., Lillehoj E.P., Kim K.C.Biochem. Biophys. Res. Commun. 310:341-346(2003) DF3/MUC1 signaling in multiple myeloma cells is regulated by interleukin-7.Li Y., Chen W., Ren J., Yu W.H., Li Q., Yoshida K., Kufe D.Cancer Biol. Ther. 2:187-193(2003) MUC1 cytoplasmic domain coactivates Wnt target gene transcription and confers transformation.Huang L., Ren J., Chen D., Li Y., Kharbanda S., Kufe D.Cancer Biol. Ther. 2:702-706(2003) Tumor necrosis factor-alpha converting enzyme/ADAM 17 mediates MUC1 shedding.Thathiah A., Blobel C.P., Carson D.D.J. Biol. Chem. 278:3386-3394(2003) Nuclear association of the cytoplasmic tail of MUC1 and beta-catenin.Wen Y., Caffrey T.C., Wheelock M.J., Johnson K.R., Hollingsworth M.A.J. Biol. Chem. 278:38029-38039(2003) Heregulin targets gamma-catenin to the nucleolus by a mechanism dependent on the DF3/MUC1 oncoprotein.Li Y., Yu W.-H., Ren J., Chen W., Huang L., Kharbanda S., Loda M., Kufe D.Mol. Cancer Res. 1:765-775(2003) MUC1 membrane trafficking is modulated by multiple interactions.Kinlough C.L., Poland P.A., Bruns J.B., Harkleroad K.L., Hughey R.P.J. Biol. Chem. 279:53071-53077(2004) Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.Wei X., Xu H., Kufe D.Cancer Cell 7:167-178(2005) MUC1 oncoprotein blocks glycogen synthase kinase 3beta-mediated phosphorylation and degradation of beta-catenin.Huang L., Chen D., Liu D., Yin L., Kharbanda S., Kufe D.Cancer Res. 65:10413-10422(2005) ErratumKinlough C.L., Poland P.A., Bruns J.B., Harkleroad K.L., Hughey R.P.J. Biol. Chem. 280:28827-28827(2005) Transmembrane and secreted MUC1 probes show trafficking-dependent changes in O-glycan core profiles.Engelmann K., Kinlough C.L., Muller S., Razawi H., Baldus S.E., Hughey R.P., Hanisch F.-G.Glycobiology 15:1111-1124(2005) MUC1 (CD227) interacts with lck tyrosine kinase in Jurkat lymphoma cells and normal T cells.Mukherjee P., Tinder T.L., Basu G.D., Gendler S.J.J. Leukoc. Biol. 77:90-99(2005) Recycling of MUC1 is dependent on its palmitoylation.Kinlough C.L., McMahan R.J., Poland P.A., Bruns J.B., Harkleroad K.L., Stremple R.J., Kashlan O.B., Weixel K.M., Weisz O.A., Hughey R.P.J. Biol. Chem. 281:12112-12122(2006) MUC1 oncoprotein stabilizes and activates estrogen receptor alpha.Wei X., Xu H., Kufe D.Mol. Cell 21:295-305(2006) MUC1 inhibits cell proliferation by a beta-catenin-dependent mechanism.Lillehoj E.P., Lu W., Kiser T., Goldblum S.E., Kim K.C.Biochim. Biophys. Acta 1773:1028-1038(2007) Human mucin 1 oncoprotein represses transcription of the p53 tumor suppressor gene.Wei X., Xu H., Kufe D.Cancer Res. 67:1853-1858(2007) Platelet-derived growth factor receptor beta-mediated phosphorylation of MUC1 enhances invasiveness in pancreatic adenocarcinoma cells.Singh P.K., Wen Y., Swanson B.J., Shanmugam K., Kazlauskas A., Cerny R.L., Gendler S.J., Hollingsworth M.A.Cancer Res. 67:5201-5210(2007) MUC1 is a novel regulator of ErbB1 receptor trafficking.Pochampalli M.R., el Bejjani R.M., Schroeder J.A.Oncogene 26:1693-1701(2007) CA 15-3 uses and limitation as a biomarker for breast cancer.Duffy M.J., Evoy D., McDermott E.W.Clin. Chim. Acta 411:1869-1874(2010) Mutations causing medullary cystic kidney disease type 1 lie in a large VNTR in MUC1 missed by massively parallel sequencing.Kirby A., Gnirke A., Jaffe D.B., Baresova V., Pochet N., Blumenstiel B., Ye C., Aird D., Stevens C., Robinson J.T., Cabili M.N., Gat-Viks I., Kelliher E., Daza R., DeFelice M., Hulkova H., Sovova J., Vylet'al P., Antignac C., Guttman M., Handsaker R.E., Perrin D., Steelman S., Sigurdsson S., Scheinman S.J., Sougnez C., Cibulskis K., Parkin M., Green T., Rossin E., Zody M.C., Xavier R.J., Pollak M.R., Alper S.L., Lindblad-Toh K., Gabriel S., Hart P.S., Regev A., Nusbaum C., Kmoch S., Bleyer A.J., Lander E.S., Daly M.J.Nat. Genet. 45:299-303(2013) N-glycosylation of the MUC1 mucin in epithelial cells and secretions.Parry S., Hanisch F.G., Leir S.H., Sutton-Smith M., Morris H.R., Dell A., Harris A.Glycobiology 16:623-634(2006) +Additional computationally mapped references.<p>Provides general information on the entry.

NCBI and Uniprot Product Information

• NCBI GI #: 67189007
• NCBI GeneID: 4582
• NCBI Accession #: NP_001018016.1
• NCBI GenBank Nucleotide #: NM_001018016.2
• UniProt Accession #: P15941; P13931; P15942; P17626; A5YRV1; A6ZID9; A6ZIE0; B1AVQ8; B1AVR0; B6ECA1; E7ESE5; E7EUG9
• Molecular Weight: 31.2kD
• NCBI Official Full Name: mucin-1 isoform 2
• NCBI Official Synonym Full Names: mucin 1, cell surface associated
• NCBI Official Symbol: MUC1
• NCBI Official Synonym Symbols: EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
• NCBI Protein Information: mucin-1
• UniProt Protein Name: Mucin-1
• Protein Family: Mucin
• UniProt Gene Name: MUC1
• UniProt Synonym Gene Names: PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta
• UniProt Entry Name: MUC1_HUMAN