Rabbit anti-Mouse, Human Camk2d Polyclonal Antibody | anti-Camk2d antibody

Camk2d, ID (Camk2d, Kiaa4163, Calcium/calmodulin-dependent protein kinase type II subunit delta) (Azide free) (HRP)

Cat. Num. AAA6280621

• Gene Names: Camk2d; CaMK II; [d]-CaMKII; 2810011D23Rik; 8030469K03Rik
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Camk2d; Polyclonal Antibody; ID (Camk2d; Kiaa4163; Calcium/calmodulin-dependent protein kinase type II subunit delta) (Azide free) (HRP); Calcium/calmodulin-dependent protein kinase type II subunit delta; anti-Camk2d antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Camk2d. Species crossreactivity: human
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Camk2d antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 221-250 in the central region of mouse Camk2d, UniProt Accession #Q6PHZ2. Species sequence homology: zebrafish, rat, bovine, chicken, porcine, rabbit, xenopus
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS6002199. This demonstrates MBS6002199 detected the Camk2d protein (arrow).)

Western Blot (WB)

(Western Blot analysis of Camk2d (arrow) usingMBS6002199. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Camk2d gene.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using MBS6002199 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6002199 for immunohistochemistry.)

Product Categories/Family for anti-Camk2d antibody

Antibodies; Protein Kinases

References

Martinez-Pena y Valenzuela, I., et al. J. Neurosci. 30(37):12455-12465(2010). Toko, H., et al. Circulation 122(9):891-899(2010). Kushnir, A., et al. Proc. Natl. Acad. Sci. U.S.A. 107(22):10274-10279(2010). Mangmool, S., et al. J. Cell Biol. 189(3):573-587(2010). Koval, O.M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(11):4996-5000(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 70906477
• NCBI GeneID: 108058
• NCBI Accession #: NP_001020609.1; NP_076302.1
• NCBI GenBank Nucleotide #: NM_001025438.2
• UniProt Accession #: Q6PHZ2; Q3UF87; Q3UQH9; Q5DTK4; Q8CAC5; Q9CZE2
• Molecular Weight: 56,369 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit delta isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II, delta
• NCBI Official Symbol: Camk2d
• NCBI Official Synonym Symbols: CaMK II; [d]-CaMKII; 2810011D23Rik; 8030469K03Rik
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit delta; caMK-II subunit delta; caM kinase II subunit delta
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit delta
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: Camk2d
• UniProt Synonym Gene Names: Kiaa4163; CaM kinase II subunit delta; CaMK-II subunit delta
• UniProt Entry Name: KCC2D_MOUSE