Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2 (Phospho-Thr286) antibody.)
Rabbit CaMK2 Polyclonal Antibody | anti-CaMK2 antibody
CaMK2 antibody (Thr286)
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
CaMK2 antibody was purified by affinity chromatography
Synonyms
CaMK2; Polyclonal Antibody; CaMK2 antibody (Thr286); Polyclonal CaMK2; Anti-CaMK2; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK-II alpha subunit; CaMK-II subunit alpha; CAMK2A; CAMKA; CaMKII; CaMKIINalpha; KCC2A; KIAA0968; anti-CaMK2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
CaMK2 antibody (Thr286) detects endogenous levels of CaMK2 only when phosphorylated at threonine 286
Purity/Purification
CaMK2 antibody was purified by affinity chromatography
Form/Format
Supplied in liquid format, PBS buffer, pH 7.4, 150mM NaCl with added preservative 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-CaMK2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:100 - 1:500
ELISA: 1:10000
IHC: 1:50 - 1:100
IF: 1:100 - 1:500
ELISA: 1:10000
Biological Significance
CaMK2 is a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
Cross-Reactivity
Mouse, Rat - Reactive with proteins phosphorylated as follows: Human: Thr286/ Thr287, Mouse: Thr286/ Thr287, Rat: Thr286/ Thr287
Immunogen
CaMK2 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human CaMK2 around the phosphorylation site of threonine 286 (Q-E-TP-V-D) as the immunogen
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated freeze thaw cycles
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2 (Phospho-Thr286) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2 (Phospho-Thr286) antibody.)
Immunofluorescence (IF)
()
Immunofluorescence (IF)
()
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using CaMK2 (Phospho-Thr286) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using CaMK2 (Phospho-Thr286) antibody.)
Related Product Information for anti-CaMK2 antibody
Rabbit polyclonal CaMK2 antibody (Thr286)
Product Categories/Family for anti-CaMK2 antibody
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Product Notes
The CaMK2 (Catalog #AAA5300289) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CaMK2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB). WB: 1:500 - 1:1000 IHC: 1:50 - 1:100 IF: 1:100 - 1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the CaMK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CaMK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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