Western Blot (WB)
(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Jurkat Cells; Lane3: Mouse Kidney Tissue.)
Rabbit anti-Human Calumenin Polyclonal Antibody | anti-CALU antibody
Polyclonal Antibody to Calumenin (CALU)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- K PTEKKDRVHH EPQLSDKVHN DAQSFDYDHD AFLGAEEAKT FDQLTPEESK ERLGKIVSKI DGDKDGFVTV DELKDWIKFA QKRWIYEDVE RQWKGHDLNE DGLVSWEEYK NATYGYVLDD PDPDDGFNYK QMMVRDERRF KMADKDGDLI ATKEEFTAFL HPEEYDYMKD IVVQETMEDI DKNADGFIDL EEYIGDMYSH DGNTDEPEWV KTEREQFVEF RDKNRDGKMD KEETKDWILP SDYDHAEAEA RHLVYESDQN KDGKLTKEEI VDKYDLFVGS QATDFGEALV RHDEF
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Jurkat Cells; Lane3: Mouse Kidney Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Jurkat Cells; Lane3: Mouse Kidney Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CALU, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CALU, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: CALU knockout Jurkat cell lysate; Predicted MW: 37kd Observed MW: 37kd Primary Ab: 3ug/ml Rabbit Anti-Human CALU Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: CALU knockout Jurkat cell lysate; Predicted MW: 37kd Observed MW: 37kd Primary Ab: 3ug/ml Rabbit Anti-Human CALU Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]
Uniprot Description
CALU: Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity. Belongs to the CREC family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Calcium-binding; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7q32.1
Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; extracellular region; Golgi apparatus; melanosome; membrane; sarcoplasmic reticulum lumen
Molecular Function: calcium ion binding; protein binding
Biological Process: cellular protein metabolic process; post-translational protein modification
Research Articles on CALU
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Product Notes
The CALU calu (Catalog #AAA2026032) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Calumenin (CALU) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calumenin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CALU calu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- K PTEKKDRVHH EPQLSDKVHN DAQSFDYDHD AFLGAEEAKT FDQLTPEESK ERLGKIVSKI DGDKDGFVTV DELKDWIKFA QKRWIYEDVE RQWKGHDLNE DGLVSWEEYK NATYGYVLDD PDPDDGFNYK QMMVRDERRF KMADKDGDLI ATKEEFTAFL HPEEYDYMKD IVVQETMEDI DKNADGFIDL EEYIGDMYSH DGNTDEPEWV KTEREQFVEF RDKNRDGKMD KEETKDWILP SDYDHAEAEA RHLVYESDQN KDGKLTKEEI VDKYDLFVGS QATDFGEALV RHDEF. It is sometimes possible for the material contained within the vial of "Calumenin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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