Rabbit Calpain 2 Polyclonal Antibody | anti-CAPN2 antibody

Calpain 2 polyclonal antibody

Cat. Num. AAA3015128

• Gene Names: CAPN2; CANP2; mCANP; CANPL2; CANPml
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE)
• Synonyms: Calpain 2; Polyclonal Antibody; Calpain 2 polyclonal antibody; Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain; CAPN2; CANPL2; anti-CAPN2 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Calpain 2 polyclonal antibody detects endogenous levels of Calpain 2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CAPN2 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:5000~1:10,000; IP:1:2000~1:8000
• Immunogen: Recombinant protein of human Calpain 2
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of Calpain 2 polyclonal antibody at 1:5000 dilutionLane1:Hela whole cell lysate(30ug)Lane2: A375 whole cell lysate(30ug)Lane3: The Skin tissue lysate of Rat(40ug)Lane4: SGC7901 whole cell lysate(40ug)Lane5: P12 whole cell lysate(40ug)Lane6: AML-12 whole cell lysate(40ug))

Immunoprecipitation (IP)

(Immunoprecipitation - Calpain 2 Polyclonal Antibody)

Related Product Information for anti-CAPN2 antibody

Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage. Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28. Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation.

NCBI and Uniprot Product Information

• NCBI GI #: 225703100
• NCBI GeneID: 824
• NCBI Accession #: NP_001139540.1
• NCBI GenBank Nucleotide #: NM_001146068.1
• UniProt Accession #: P17655; Q16738; Q6PJT3; Q8WU26; Q9HBB1; A6NDG7; B7ZA96; E7ES58
• Molecular Weight: 79,995 Da
• NCBI Official Full Name: calpain-2 catalytic subunit isoform 2
• NCBI Official Synonym Full Names: calpain 2, (m/II) large subunit
• NCBI Official Symbol: CAPN2
• NCBI Official Synonym Symbols: CANP2; mCANP; CANPL2; CANPml
• NCBI Protein Information: calpain-2 catalytic subunit; CANP 2; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain, large polypeptide L2; calpain 2, large [catalytic] subunit; calcium-activated neu
• UniProt Protein Name: Calpain-2 catalytic subunit
• Protein Family: Calpain
• UniProt Gene Name: CAPN2
• UniProt Synonym Gene Names: CANPL2; CANP 2; M-calpain
• UniProt Entry Name: CAN2_HUMAN

NCBI Description

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

CAPN2: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Motility/polarity/chemotaxis; EC 3.4.22.53

Chromosomal Location of Human Ortholog: 1q41-q42

Cellular Component: Golgi apparatus; cortical actin cytoskeleton; focal adhesion; endoplasmic reticulum; dendrite; cytoplasm; plasma membrane; pseudopodium; cytosol; nucleus; chromatin; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; cysteine-type peptidase activity

Biological Process: protein autoprocessing; blastocyst development; proteolysis involved in cellular protein catabolic process; response to hypoxia; proteolysis; myoblast fusion; regulation of cytoskeleton organization and biogenesis