Sheep Ribonuclease H ELISA Kit | RNASEH elisa kit

Sheep Ribonuclease H ELISA Kit

Cat. Num. AAA746363

• Reactivity: Sheep
• Synonyms: Ribonuclease H; Sheep Ribonuclease H ELISA Kit; RNASEH elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This RNaseH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep RNaseH. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This RNaseH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep RNaseH. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RNASEH elisa kit

Principle of the Assay: RNaseH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RNaseH antibody and an RNaseH-HRP conjugate. The assay sample and buffer are incubated together with RNaseH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNaseH concentration since RNaseH from samples and RNaseH-HRP conjugate compete for the anti-RNaseH antibody binding site. Since the number of sites is limited, as more sites are occupied by RNaseH from the sample, fewer sites are left to bind RNaseH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNaseH concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RNASEH elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 428271012
• NCBI GeneID: 14162226
• Molecular Weight: 17,479 Da
• NCBI Official Full Name: Ribonuclease H
• NCBI Official Symbol: Sta7437_3452
• NCBI Protein Information: Ribonuclease H
• UniProt Protein Name: Ribonuclease H
• Protein Family: Ribonuclease
• UniProt Gene Name: rnhA
• UniProt Synonym Gene Names: Sta7437_3452; RNase H
• UniProt Entry Name: K9XWP3_STAC7

Uniprot Description

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.