Rabbit anti-Human Calpain 10 Polyclonal Antibody | anti-CAPN10 antibody

Calpain 10 (Calpain-10, Calcium Activated Neutral Protease, Calcium Activated Neutral Proteinase 10, CANP 10, CAPN 10, CAPN10, EC 3.4.22, KIAA1845, NIDDM1) (FITC)

Cat. Num. AAA6372182

• Gene Names: CAPN10; CANP10; NIDDM1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Calpain 10; Polyclonal Antibody; Calpain 10 (Calpain-10; Calcium Activated Neutral Protease; Calcium Activated Neutral Proteinase 10; CANP 10; CAPN 10; CAPN10; EC 3.4.22; KIAA1845; NIDDM1) (FITC); anti-CAPN10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CAPN10.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CAPN10 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CAPN10, aa1-672 (NP_075571.1).
• Immunogen Sequence: MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQLLHLKDQCLISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQCILLLRIQNPWGRRCWQGLWREGGEGWSQVDAAVASELLSQLQEGEFWVEEEEFLREFDELTVGYPVTEAGHLQSLYTERLLCHTRALPGAWVKGQSAGGCRNNSGFPSNPKFWLRVSEPSEVYIAVLQRSRLHAADWAGRARALVGDSHTSWSPASIPGKHYQAVGLHLWKVEKRRVNLPRVLSMPPVAGTACHAYDREVHLRCELSPGYYLAVPSTFLKDAPGEFLLRVFSTGRVSLSAIRAVAKNTTPGAALPAGEWGTVQLRGSWRVGQTAGGSRNFASYPTNPCFPFSVPEGPGPRCVRITLHQHCRPSDTEFHPIGFHIFQVPEGGRSQDAPPLLLQEPLLSCVPHRYAQEVSRLCLLPAGTYKVVPSTYLPDTEGAFTVTIATRIDRPSIHSQEMLGQFLQEVSVMAVMKT
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CAPN10 expression in transfected 293T cell line by CAPN10 polyclonal antibody. Lane 1: CAPN10 transfected lysate (74.9kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-CAPN10 antibody

Antibodies; Calpain

NCBI and Uniprot Product Information

• NCBI GI #: 13186302
• NCBI GeneID: 11132
• NCBI Accession #: NP_075571.1
• NCBI GenBank Nucleotide #: NM_023083.2
• UniProt Accession #: Q9HC96; Q4ZFV1; Q8NCD4; Q96IG4; Q96JI2; Q9HC89; Q9HC90; Q9HC91; Q9HC92; Q9HC93; Q9HC94; A8MVS7
• Molecular Weight: 74,952 Da
• NCBI Official Full Name: calpain-10 isoform a
• NCBI Official Synonym Full Names: calpain 10
• NCBI Official Symbol: CAPN10
• NCBI Official Synonym Symbols: CANP10; NIDDM1
• NCBI Protein Information: calpain-10; calpain-like protease CAPN10; calcium-activated neutral proteinase 10
• UniProt Protein Name: Calpain-10
• Protein Family: Calpain
• UniProt Gene Name: CAPN10
• UniProt Synonym Gene Names: KIAA1845; CANP 10
• UniProt Entry Name: CAN10_HUMAN

NCBI Description

Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

CAPN10: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Defects in CAPN10 are a cause of susceptibility to diabetes mellitus non-insulin-dependent type 1 (NIDDM1). It is a multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the peptidase C2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.-; Protease; Mitochondrial

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: mitochondrion; cell; plasma membrane; cytosol

Molecular Function: SNARE binding; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity

Biological Process: positive regulation of glucose import; cellular response to insulin stimulus; positive regulation of intracellular transport; actin cytoskeleton reorganization; positive regulation of insulin secretion; proteolysis; cell structure disassembly during apoptosis

Disease: Diabetes Mellitus, Noninsulin-dependent, 1