Western Blot (WB)
(Western blot analysis of CALCOCO1 expression in MCF7 (A), A549 (B), mouse lung (C), mouse heart (D) whole cell lysates.)
Rabbit anti-Human CALCOCO1 Polyclonal Antibody | anti-CALCOCO1 antibody
Anti-CALCOCO1 Antibody
Western Blot (WB)
(Western blot analysis of CALCOCO1 expression in MCF7 (A), A549 (B), mouse lung (C), mouse heart (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of CALCOCO1 expression in MCF7 (A), A549 (B), mouse lung (C), mouse heart (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CALCOCO1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CALCOCO1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of CALCOCO1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of CALCOCO1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
Uniprot Description
CALCOCO1: Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1- mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions. Belongs to the CALCOCO family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: cytoplasm; intracellular membrane-bound organelle; nuclear chromatin; nucleus
Molecular Function: beta-catenin binding; chromatin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein C-terminus binding; sequence-specific DNA binding; transcription coactivator activity; transcription cofactor activity
Biological Process: positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; signal transduction; steroid hormone receptor signaling pathway; transcription, DNA-dependent; Wnt receptor signaling pathway
Research Articles on CALCOCO1
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Product Notes
The CALCOCO1 calcoco1 (Catalog #AAA8245381) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CALCOCO1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CALCOCO1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/100. Researchers should empirically determine the suitability of the CALCOCO1 calcoco1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CALCOCO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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