Western Blot (WB)
(Western blot analysis of extracts from Rat brain, using Calcium Pump PMCA4 ATPase Antibody. The lane on the left was treated with blocking peptide.)
Rabbit Calcium Pump PMCA4 ATPase Polyclonal Antibody | anti-ATP2B4 antibody
Calcium Pump PMCA4 ATPase Antibody
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Rabbit(88%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Rabbit(88%), Dog(100%)
IF: 1:100-1:500
ICC: 1:100-1:500
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from Rat brain, using Calcium Pump PMCA4 ATPase Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Rat brain, using Calcium Pump PMCA4 ATPase Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612085 at 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612085 at 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612085 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612085 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612085 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612085 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612085 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612085 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Subcellular Location: Cell membrane>Multi-pass membrane protein. Cell projection>Cilium>Flagellum membrane>Multi-pass membrane protein.
Tissue Specificity: Isoform XB is the most abundant isoform and is expressed ubiquitously. Isoforms containing segment Z have only been detected in heart, while isoforms containing segment a have been found in heart, stomach and brain cortex.
Subunit Structure: Interacts with PDZD11 (PubMed:12763866). Interacts with SLC35G1 and STIM1 (PubMed:22084111). Interacts with calmodulin (PubMed:2963820, PubMed:10493800).
Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)138kDa.
NCBI Description
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
PMCA4: a plasma membrane calcium pump. A magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Eight splice-variant isoforms have been described.
Protein type: Channel, calcium; EC 3.6.3.8; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: basolateral plasma membrane; caveola; integral component of plasma membrane; intracellular membrane-bound organelle; membrane; neuron projection; plasma membrane; protein complex; T-tubule; Z disc
Molecular Function: ATP binding; calcium-transporting ATPase activity; calmodulin binding; metal ion binding; nitric-oxide synthase binding; nitric-oxide synthase inhibitor activity; PDZ domain binding; protein binding; protein kinase binding; protein phosphatase 2B binding; sodium channel regulator activity
Biological Process: calcium ion export; calcium ion import across plasma membrane; cellular calcium ion homeostasis; cellular response to epinephrine stimulus; hippocampus development; negative regulation of adrenergic receptor signaling pathway involved in heart process; negative regulation of arginine catabolic process; negative regulation of nitric oxide biosynthetic process; negative regulation of nitric oxide mediated signal transduction; negative regulation of nitric-oxide synthase activity; negative regulation of peptidyl-cysteine S-nitrosylation; negative regulation of the force of heart contraction; neural retina development; positive regulation of cAMP-dependent protein kinase activity; positive regulation of peptidyl-serine phosphorylation; regulation of cell cycle G1/S phase transition; regulation of sodium ion transmembrane transport; regulation of transcription from RNA polymerase II promoter; response to hydrostatic pressure; sperm motility; spermatogenesis
Research Articles on ATP2B4
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Product Notes
The ATP2B4 atp2b4 (Catalog #AAA9612085) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calcium Pump PMCA4 ATPase Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Rabbit(88%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Calcium Pump PMCA4 ATPase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:500 ICC: 1:100-1:500 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the ATP2B4 atp2b4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcium Pump PMCA4 ATPase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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