Rabbit Calcitonin Polyclonal Antibody | anti-CALCA antibody

Calcitonin Polyclonal Antibody

Cat. Num. AAA3006657

• Gene Names: CALCA; CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I; CGRP-alpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Calcitonin; Polyclonal Antibody; Calcitonin Polyclonal Antibody; Katacalcin; Calcitonin carboxyl-terminal peptide; CCP; PDN-21; CALCA; CALC1; anti-CALCA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Calcitonin polyclonal antibody detects endogenous levels of Calcitonin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 141

• Applicable Applications for anti-CALCA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human Calcitonin.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Calcitonin polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Mouse heart tissue lysateLane3:Rat heart tissue lysate)

Related Product Information for anti-CALCA antibody

Calcitonin is a 32 amino acid polypeptide hormone that preserves skeletal integrity and reduces blood calcium levels by decreasing osteoclast activity in bones, calcium and phosphate reabsorption by kidney tubules and calcium absorption by the intestines. The secretion of Calcitonin from the thyroid is regulated in part by estrogen, which increases Calcitonin mRNA levels. The Calcitonin gene, CALCA, undergoes tissue-specific RNA alternative splicing, resulting in the production of different mRNA transcripts. One transcript encodes procalcitonin as well as both calcium-lowering processed active polypeptides, Calcitonin and katacalcin. An alternative transcript of CALCA encodes the precursor for the neuropeptide referred to as Calcitonin gene-related peptide 1, also designated CGRP1 or alpha-CGRP. CGRP is a widely distributed vasodilatory peptide. Calcitonin and katacalcin are produced primarily in the thyroid, while CGRP is produced in neuronal cells. A second CGRP related gene, CALCB, thought to be derived from an gene duplication event, has been identified in mouse, rat and human. Unlike CALCA, CALCB is not subject to alternative splicing and encodes a single transcript designated CGRP2 or beta-CGRP. Mature CGRP1 and CGRP2 share significant sequence identity at the protein level differing by only 1-3 amino acid residues, depending on the species.

NCBI and Uniprot Product Information

• NCBI GI #: 76880484
• NCBI GeneID: 796
• NCBI Accession #: NP_001029124.1
• NCBI GenBank Nucleotide #: NP_001029124.1
• UniProt Accession #: P01258; P06881
• Molecular Weight: ~ 19kDa
• NCBI Official Full Name: calcitonin isoform CT preproprotein
• NCBI Official Synonym Full Names: calcitonin related polypeptide alpha
• NCBI Official Symbol: CALCA
• NCBI Official Synonym Symbols: CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I; CGRP-alpha
• NCBI Protein Information: calcitonin; calcitonin gene-related peptide 1
• UniProt Protein Name: Calcitonin
• Protein Family: Calcitonin
• UniProt Gene Name: CALCA
• UniProt Synonym Gene Names: CALC1; CCP
• UniProt Entry Name: CALC_HUMAN

NCBI Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

Uniprot Description

CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space; cell soma; cytoplasm; extracellular region; terminal button; nucleus

Molecular Function: identical protein binding; protein binding; calcitonin receptor binding; hormone activity; protein complex binding; receptor binding

Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; protein amino acid phosphorylation; G-protein signaling, adenylate cyclase activating pathway; positive regulation of interleukin-8 production; monocyte chemotaxis; elevation of cytosolic calcium ion concentration; leukocyte adhesion; cell-cell signaling; vasculature development; neuropeptide signaling pathway; negative regulation of smooth muscle contraction; receptor internalization; regulation of blood pressure; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; negative regulation of blood pressure; positive regulation of adenylate cyclase activity; positive regulation of macrophage differentiation; negative regulation of bone resorption; detection of temperature stimulus involved in sensory perception of pain; inflammatory response; negative regulation of neurological process; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; regulation of inflammatory response; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation