<
Immunofluorescence (IF)
(Immunofluorescence staining of CALB1 in 293 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CALB1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
Rabbit Calbindin Polyclonal Antibody | anti-CALB1 antibody
Anti-Calbindin Antibody, Rabbit Polyclonal
Gene Names
CALB1; CALB
Reactivity
HumanPredicted to React with: Rat (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Calbindin; Polyclonal Antibody; Anti-Calbindin Antibody; Rabbit Polyclonal; Calbindin Antibody; Rabbit PAb; Antigen Affinity Purified; calbindin 1; 28kDa; Anti-CALB Antibody; Anti-D-28K Antibody; anti-CALB1 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Calbindin
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CALB1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:500-1:2000
ICC/IF: 1:300-1:10,000
IP: 4-8uL/mg of lysate
IHC-P: 1:500-1:2000
ICC/IF: 1:300-1:10,000
IP: 4-8uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Calbindin
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Calbindin, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of CALB1 in 293 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CALB1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of CALB1 in 293 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CALB1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CALB1 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human CALB1 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(CALB1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate4 uL anti-CALB1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-CALB1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa)
Immunoprecipitation (IP)
(CALB1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate4 uL anti-CALB1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-CALB1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa)
Western Blot (WB)
(Anti-CALB1 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mosue kidney tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:27 kDa)
Western Blot (WB)
(Anti-CALB1 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mosue kidney tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:27 kDa)
Product Categories/Family for anti-CALB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
261
NCBI Official Full Name
calbindin
NCBI Official Synonym Full Names
calbindin 1, 28kDa
NCBI Official Symbol
CALB1
NCBI Official Synonym Symbols
CALB
NCBI Protein Information
calbindin; D-28K; calbindin D28; RTVL-H protein; calbindin 1, (28kD); vitamin D-dependent calcium-binding protein, avian-type
UniProt Protein Name
Calbindin
Protein Family
UniProt Gene Name
CALB1
UniProt Synonym Gene Names
CAB27
UniProt Entry Name
CALB1_HUMAN
Similar Products
Product Notes
The CALB1 calb1 (Catalog #AAA8101717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Calbindin Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Calbindin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:500-1:2000 ICC/IF: 1:300-1:10,000 IP: 4-8uL/mg of lysate. Researchers should empirically determine the suitability of the CALB1 calb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calbindin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
