Calbindin 1, Mouse Polyclonal Antibody | anti-CALB1 antibody
Anti - Calbindin 1, Mouse (CABP28K, CALB1, Spot35 protein, Cholecalcin)
Gene Names
CALB1; CALB
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity purified over the control-peptide Sepharose
Synonyms
Calbindin 1; Mouse; Polyclonal Antibody; Anti - Calbindin 1; Mouse (CABP28K; CALB1; Spot35 protein; Cholecalcin); anti-CALB1 antibody
Host
Host: Rabbit
Source: Mouse
Source: Mouse
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
The peptide is 95% conserved in rat, human, rabbit, chicken, bovine D28K. No significant sequence homology is seen with D9K, calretinin or other CABPs.
Purity/Purification
Affinity purified over the control-peptide Sepharose
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Sequence Length
261
Applicable Applications for anti-CALB1 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. D28K is ~28kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1ug of affinity purified IgG or 1ul antiserum) to confirm antibody specificity.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. D28K is ~28kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1ug of affinity purified IgG or 1ul antiserum) to confirm antibody specificity.
Optimal dilutions to be determined by the researcher.
Immunogen
A 20-aa peptide sequence, within the C-terminal, non-calcium binding domain of mouse D28K (1) was synthesized, coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-CALB1 antibody
References
• 1. Nordquist DT et al (1988) J. Neurochem. 8, 4780; Takeda T et al (1994) BBRC 204, 889; Wood TL et al (1988) DNA 7, 585; Parmentier M et al (1987) Eur. J. Biochem. 170, 207; Gross MD et al (1988) JBC 263, 14426; Lomri N et al (1989) Gene 80, 87-98.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,025 Da
NCBI Official Full Name
calbindin
NCBI Official Synonym Full Names
calbindin 1, 28kDa
NCBI Official Symbol
CALB1
NCBI Official Synonym Symbols
CALB
NCBI Protein Information
calbindin; D-28K; calbindin D28; RTVL-H protein; OTTHUMP00000166027; OTTHUMP00000225441; calbindin 1, (28kD); vitamin D-dependent calcium-binding protein, avian-type
UniProt Protein Name
Calbindin
Protein Family
UniProt Gene Name
CALB1
UniProt Synonym Gene Names
CAB27
UniProt Entry Name
CALB1_HUMAN
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Product Notes
The CALB1 calb1 (Catalog #AAA617430) is an Antibody produced from Host: Rabbit Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti - Calbindin 1, Mouse (CABP28K, CALB1, Spot35 protein, Cholecalcin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Calbindin 1, Mouse can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. D28K is ~28kD. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1ug of affinity purified IgG or 1ul antiserum) to confirm antibody specificity. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the CALB1 calb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calbindin 1, Mouse, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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