Western Blot (WB)
(Western blot analysis of lysates from COLO cells, using GPR31 Antibody. The lane on the right is blocked with the synthesized peptide.)
Rabbit anti-Human GPR31 Polyclonal Antibody | anti-GPR31 antibody
GPR31 Antibody
Gene Names
GPR31; HETER; HETER1; 12-HETER
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
GPR31; Polyclonal Antibody; GPR31 Antibody; anti-GPR31 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
GPR31 Antibody detects endogenous levels of total GPR31 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
319
Applicable Applications for anti-GPR31 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:1000
ELISA: 1:1000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR31.
Immunogen Range
181-230
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysates from COLO cells, using GPR31 Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from COLO cells, using GPR31 Antibody. The lane on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35 kDa
NCBI Official Full Name
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor
NCBI Official Synonym Full Names
G protein-coupled receptor 31
NCBI Official Symbol
GPR31
NCBI Official Synonym Symbols
HETER; HETER1; 12-HETER
NCBI Protein Information
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor
UniProt Protein Name
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor
Protein Family
UniProt Gene Name
GPR31
UniProt Synonym Gene Names
12-(S)-HETE receptor; 12-HETER
Uniprot Description
High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.
Research Articles on GPR31
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Product Notes
The GPR31 gpr31 (Catalog #AAA9508059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR31 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPR31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the GPR31 gpr31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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