Western Blot (WB)
(Anti-CADM3 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse Brain Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:45 kDa)
Rabbit anti-Mouse CADM3 Polyclonal Antibody | anti-CADM3 antibody
Anti-CADM3 Antibody, Rabbit Polyclonal
Gene Names
Cadm3; BIgR; Necl1; Tsll1; Igsf4b; Necl-1; SynCAM3
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
CADM3; Polyclonal Antibody; Anti-CADM3 Antibody; Rabbit Polyclonal; CADM3/NECL1/IGSF4B Antibody; Rabbit PAb; Antigen Affinity Purified; cell adhesion molecule 3; Anti-BIgR Antibody; Anti-Igsf4b Antibody; Anti-Necl-1 Antibody; Anti-Necl1 Antibody; Anti-SynCAM3 Antibody; Anti-Tsll1 Antibody; anti-CADM3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse CADM3/NECL1/IGSF4B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CADM3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant Mouse CADM3/NECL1/IGSF4B Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CADM3/NECL1/IGSF4B (rM CADM3/NECL1/IGSF4B). CADM3/NECL1/IGSF4B specific IgG was purified by Mouse CADM3/NECL1/IGSF4B affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CADM3 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse Brain Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:45 kDa)
Western Blot (WB)
(Anti-CADM3 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse Brain Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:45 kDa)
Related Product Information for anti-CADM3 antibody
Cell Adhesion Molecules (CAMs) are proteins located on the cell surface involved with the binding with other cells or with the extracellular matrix (ECM) in the process called cell adhesion. These proteins are typically transmembrane receptors and are composed of three domains: an intracellular domain that interacts with the cytoskeleton, a transmembrane domain, and an extracellular domain that interacts either with other CAMs of the same kind (homophilic binding) or with other CAMs or the extracellular matrix (heterophilic binding). Cell adhesion molecule 3, also known as Immunoglobulin superfamily member 4B, CADM3, and NECL1, is a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule which has Ca(2+)-independent homo-or heterophilic cell-cell adhesion activity and plays an important role in the formation of synapses, axon bundles and myelinated axons. Isoform 1 of CADM3 is expressed mainly in adult and fetal brain. Isoform 2 of CADM3 is highly expressed in adult brain and weakly expressed in placenta. In brain, Isoform 2 is highly expressed in cerebellum. CADM3 is involved in the cell-cell adhesion. It has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. The interaction with EPB41L1 may regulate structure or function of cell-cell junctions. CADM3 may act as a tumor suppressor in glioma and loss of it in glioma may be caused by histone deacetylation.
References
Dong X, et al. (2006) Crystal structure of the V domain of human Nectin-like molecule-1/Syncam3/Tsll1/Igsf4b, a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule. J Biol Chem. 281(15): 10610-7.Gao J, et al. (2008) Nectin-like molecule 1 is a glycoprotein with a single N-glycosylation site at N290KS which influences its adhesion activity. Biochim Biophys Acta. 1778(6): 1429-35.Gao J, et al. (2009) Loss of NECL1, a novel tumor suppressor, can be restored in glioma by HDAC inhibitor-Trichostatin A through Sp1 binding site. Glia. 57(9): 989-99.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,964 Da
NCBI Official Full Name
cell adhesion molecule 3
NCBI Official Synonym Full Names
cell adhesion molecule 3
NCBI Official Symbol
Cadm3
NCBI Official Synonym Symbols
BIgR; Necl1; Tsll1; Igsf4b; Necl-1; SynCAM3
NCBI Protein Information
cell adhesion molecule 3
UniProt Protein Name
Cell adhesion molecule 3
Protein Family
UniProt Gene Name
Cadm3
UniProt Synonym Gene Names
Igsf4b; Necl1; Syncam3; Tsll1; IgSF4B; NECL-1
UniProt Entry Name
CADM3_MOUSE
Uniprot Description
CADM3: Involved in the cell-cell adhesion. Has both calcium- independent homophilic cell-cell adhesion activity and calcium- independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. Interaction with EPB41L1 may regulate structure or function of cell-cell junctions. Belongs to the nectin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral
Cellular Component: membrane; plasma membrane; integral to membrane; intercellular junction; cell junction
Molecular Function: protein binding; protein homodimerization activity
Biological Process: heterophilic cell adhesion; protein localization; homophilic cell adhesion; cell adhesion
Research Articles on CADM3
Similar Products
Product Notes
The CADM3 cadm3 (Catalog #AAA8111636) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CADM3 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CADM3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the CADM3 cadm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CADM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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